Universität zu Köln

Sriramachandran, Annie M., Meyer-Teschendorf, Katrin, Pabst, Stefan ORCID: 0000-0003-1073-4246, Ulrich, Helle D. ORCID: 0000-0003-0431-2223, Gehring, Niels H., Hofmann, Kay, Praefcke, Gerrit J. K. and Dohmen, R. Jurgen ORCID: 0000-0002-5756-6780 (2019). Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain. Nat. Commun., 10. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Modification with SUMO regulates many eukaryotic proteins. Down-regulation of sumoylated forms of proteins involves either their desumoylation, and hence recycling of the unmodified form, or their proteolytic targeting by ubiquitin ligases that recognize their SUMO modification (termed STUbL or ULS). STUbL enzymes such as Uls1 and Slx5-Slx8 in budding yeast or RNF4 and Arkadia/RNF111 in humans bear multiple SUMO interaction motifs to recognize substrates carrying poly-SUMO chains. Using yeast as experimental system and isothermal titration calorimetry, we here show that Arkadia specifically selects substrates carrying SUMO1-capped SUMO2/3 hybrid conjugates and targets them for proteasomal degradation. Our data suggest that a SUMO1-specific binding site in Arkadia with sequence similarity to a SUMO1-binding site in DPP9 is required for targeting endogenous hybrid SUMO conjugates and PML nuclear bodies in human cells. We thus characterize Arkadia as a STUbL with a preference for substrate proteins marked with distinct hybrid SUMO chains.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sriramachandran, Annie M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer-Teschendorf, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Pabst, Stefan UNSPECIFIED orcid.org/0000-0003-1073-4246 UNSPECIFIED Ulrich, Helle D. UNSPECIFIED orcid.org/0000-0003-0431-2223 UNSPECIFIED Gehring, Niels H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Kay UNSPECIFIED UNSPECIFIED UNSPECIFIED Praefcke, Gerrit J. K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dohmen, R. Jurgen UNSPECIFIED orcid.org/0000-0002-5756-6780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144278
DOI:	10.1038/s41467-019-11549-3
Journal or Publication Title:	Nat. Commun.
Volume:	10
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN MODIFICATION; RECOGNITION; MODIFIER; PML; MOTIF; SUMOYLATION; RNF4 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14427