Universität zu Köln

Xiao, Yu ORCID: 0000-0003-2518-9594, Stegmann, Martin, Han, Zhifu, DeFalco, Thomas A., Parys, Katarzyna, Xu, Li, Belkhadir, Youssef ORCID: 0000-0001-7149-7139, Zipfel, Cyril and Chai, Jijie (2019). Mechanisms of RALF peptide perception by a heterotypic receptor complex. Nature, 572 (7768). S. 270 - 289. LONDON: NATURE PUBLISHING GROUP. ISSN 1476-4687

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Abstract

Receptor kinases of the Catharanthus roseus RLK1-like (CrRLK1L) family have emerged as important regulators of plant reproduction, growth and responses to the environment(1). Endogenous RAPID ALKALINIZATION FACTOR (RALF) peptides(2) have previously been proposed as ligands for several members of the CrRLK1L family(1). However, the mechanistic basis of this perception is unknown. Here we report that RALF23 induces a complex between the CrRLK1L FERONIA (FER) and LORELEI (LRE)-LIKE GLYCOSYLPHOSPHATIDYLINOSITOL (GPI)-ANCHORED PROTEIN 1 (LLG1) to regulate immune signalling. Structural and biochemical data indicate that LLG1 (which is genetically important for RALF23 responses) and the related LLG2 directly bind RALF23 to nucleate the assembly of RALF23-LLG1-FER and RALF23-LLG2-FER heterocomplexes, respectively. A conserved N-terminal region of RALF23 is sufficient for the biochemical recognition of RALF23 by LLG1, LLG2 or LLG3, and binding assays suggest that other RALF peptides that share this conserved N-terminal region may be perceived by LLG proteins in a similar manner. Structural data also show that RALF23 recognition is governed by the conformationally flexible C-terminal sides of LLG1, LLG2 and LLG3. Our work reveals a mechanism of peptide perception in plants by GPI-anchored proteins that act together with a phylogenetically unrelated receptor kinase. This provides a molecular framework for understanding how diverse RALF peptides may regulate multiple processes, through perception by distinct heterocomplexes of CrRLK1L receptor kinases and GPI-anchored proteins of the LRE and LLG family.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xiao, Yu UNSPECIFIED orcid.org/0000-0003-2518-9594 UNSPECIFIED Stegmann, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Han, Zhifu UNSPECIFIED UNSPECIFIED UNSPECIFIED DeFalco, Thomas A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Parys, Katarzyna UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Belkhadir, Youssef UNSPECIFIED orcid.org/0000-0001-7149-7139 UNSPECIFIED Zipfel, Cyril UNSPECIFIED UNSPECIFIED UNSPECIFIED Chai, Jijie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144636
DOI:	10.1038/s41586-019-1409-7
Journal or Publication Title:	Nature
Volume:	572
Number:	7768
Page Range:	S. 270 - 289
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	1476-4687
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-WALL INTEGRITY; ARABIDOPSIS; FERONIA; PROTEIN; MALECTIN; KINASES; LORELEI; GROWTH; FLS2; LRX Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14463