Universität zu Köln

Shanmugarajah, Kalpana, Linka, Nicole, Graefe, Katharina, Smits, Sander H. J., Weber, Andreas P. M., Zeier, Juergen and Schmitt, Lutz ORCID: 0000-0002-1167-9819 (2019). ABCG1 contributes to suberin formation in Arabidopsis thaliana roots. Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Diffusion barriers enable plant survival under fluctuating environmental conditions. They control internal water potential and protect against biotic or abiotic stress factors. How these protective molecules are deposited to the extracellular environment is poorly understood. We here examined the role of the Arabidopsis ABC half-size transporter AtABCG1 in the formation of the extracellular root suberin layer. Quantitative analysis of extracellular long-chain fatty acids and aliphatic alcohols in the atabcgl mutants demonstrated altered root suberin composition, specifically a reduction in longer chain dicarboxylic acids, fatty alcohols and acids. Accordingly, the ATP-hydrolyzing activity of heterologous expressed and purified AtABCG1 was strongly stimulated by fatty alcohols (C-26-C-30) and fatty acids (C-24-C-30) in a chain length dependent manner. These results are a first indication for the function of AtABCG1 in the transport of longer chain aliphatic monomers from the cytoplasm to the apoplastic space during root suberin formation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shanmugarajah, Kalpana UNSPECIFIED UNSPECIFIED UNSPECIFIED Linka, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Graefe, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Smits, Sander H. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeier, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitt, Lutz UNSPECIFIED orcid.org/0000-0002-1167-9819 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144713
DOI:	10.1038/s41598-019-47916-9
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ATP-BINDING; TRANSPORTER GENE; EXINE FORMATION; BARRIERS; MUTANT; CUTIN; BIOSYNTHESIS; TRAFFICKING; METABOLISM; SECRETION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14471