Universität zu Köln

Price, Dana C., Goodenough, Ursula W., Roth, Robyn, Lee, Jae-Hyeok, Kariyawasam, Thamali, Mutwil, Marek ORCID: 0000-0002-7848-0126, Ferrari, Camilla ORCID: 0000-0002-5843-0500, Facchinelli, Fabio, Ball, Steven G., Cenci, Ugo, Chan, Cheong Xin, Wagner, Nicole E., Yoon, Hwan Su, Weber, Andreas P. M. and Bhattacharya, Debashish (2019). Analysis of an improved Cyanophora paradoxa genome assembly. DNA Res., 26 (4). S. 287 - 300. OXFORD: OXFORD UNIV PRESS. ISSN 1756-1663

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Abstract

Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-read sequences, of the ca. 100 Mb nuclear genome of the model glaucophyte Cyanophora paradoxa. We also conducted a quick-freeze deep-etch electron microscopy (QFDEEM) analysis of C. paradoxa cells to investigate glaucophyte morphology in comparison to other organisms. Using the genome data, we generated a resolved 115-taxon eukaryotic tree of life that includes a well-supported, monophyletic Archaeplastida. Analysis of muroplast peptidoglycan (PG) ultrastructure using QFDEEM shows that PG is most dense at the cleavage-furrow. Analysis of the chlamydial contribution to glaucophytes and other Archaeplastida shows that these foreign sequences likely played a key role in anaerobic glycolysis in primordial algae to alleviate ATP starvation under night-time hypoxia. The robust genome assembly of C. paradoxa significantly advances knowledge about this model species and provides a reference for exploring the panoply of traits associated with the anciently diverged glaucophyte lineage.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Price, Dana C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goodenough, Ursula W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roth, Robyn UNSPECIFIED UNSPECIFIED UNSPECIFIED Lee, Jae-Hyeok UNSPECIFIED UNSPECIFIED UNSPECIFIED Kariyawasam, Thamali UNSPECIFIED UNSPECIFIED UNSPECIFIED Mutwil, Marek UNSPECIFIED orcid.org/0000-0002-7848-0126 UNSPECIFIED Ferrari, Camilla UNSPECIFIED orcid.org/0000-0002-5843-0500 UNSPECIFIED Facchinelli, Fabio UNSPECIFIED UNSPECIFIED UNSPECIFIED Ball, Steven G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cenci, Ugo UNSPECIFIED UNSPECIFIED UNSPECIFIED Chan, Cheong Xin UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Nicole E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Yoon, Hwan Su UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bhattacharya, Debashish UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144979
DOI:	10.1093/dnares/dsz009
Journal or Publication Title:	DNA Res.
Volume:	26
Number:	4
Page Range:	S. 287 - 300
Date:	2019
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1756-1663
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RNA-SEQ DATA; CHLOROPLAST DIVISION; PHOTOSYNTHETIC EUKARYOTES; CELL-WALLS; CYANELLES; EVOLUTION; ULTRASTRUCTURE; PEPTIDOGLYCAN; ORIGIN; CYANOBACTERIAL Multiple languages Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14497