Universität zu Köln

Yu, Xiaoyu, He, Xiaoyu, Heindl, L. M., Song, Xin, Fan, Jiayan and Jia, Renbing (2019). KIF15 plays a role in promoting the tumorigenicity of melanoma. Exp. Eye Res., 185. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 1096-0007

Full text not available from this repository.

Abstract

Kinesins are a superfamily of motor proteins and are often dysregulated in many cancers. KIF15, which belongs to the kinesin-12 family, has been shown to function in many different cellular processes, including proliferation, apoptosis, differentiation and development. However, the role of KIF15 in melanoma, remains unknown. In this study, the expression levels of KIF15 in melanoma cells lines and tissues were determined via real-time PCR, immunohistochemical staining and western blot. The effect of KIF15 on tumorigenesis was evaluated by using MTT and colony information. The function of KIF15 on cell survival was detected through flow cytometry assay. Microarray assay and bioinformatics analysis were used to find the potential target of KIF15. We show that KIF15 was significantly upregulated in melanoma cells and tissues. The suppression of KIF15 in tumors significantly reduced tumor growth and increased apoptosis in A375 and OCM1 cells. Findings based on the subcutaneous xenograft model were further consistent with the in vitro results that KIF15 knockdown inhibited melanoma tumor growth in vivo. Microarray assay and bioinformatics indicated that BIRC5, CDK4 and WNT5A were three potential targets of KIF15. Taken together, our results suggest that KIF15 plays a positive role in the tumorigenicity of melanoma and it may serve as a novel diagnostic and therapeutic target for melanoma, especially uveal melanoma.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Yu, Xiaoyu UNSPECIFIED UNSPECIFIED UNSPECIFIED He, Xiaoyu UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, L. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Song, Xin UNSPECIFIED UNSPECIFIED UNSPECIFIED Fan, Jiayan UNSPECIFIED UNSPECIFIED UNSPECIFIED Jia, Renbing UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-145291
DOI:	10.1016/j.exer.2019.02.014
Journal or Publication Title:	Exp. Eye Res.
Volume:	185
Date:	2019
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	1096-0007
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SURVIVIN; EXPRESSION; APOPTOSIS; PROTEIN; WNT5A; GENE; PROLIFERATION; CYTOKINESIS; KINESIN-12; RESISTANCE Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14529