Universität zu Köln

Maleki, Hajar ORCID: 0000-0002-2813-4700, Shahbazi, Mohammad-Ali ORCID: 0000-0002-4860-3017, Montes, Susan, Hosseini, Seyed Hojjat, Eskandari, Mohammad Reza ORCID: 0000-0001-6219-7185, Zaunschirm, Stefan, Verwanger, Thomas ORCID: 0000-0003-2651-8898, Mathur, Sanjay ORCID: 0000-0003-2765-2693, Milow, Barbara, Krammer, Barbara and Huesing, Nicola (2019). Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration. ACS Appl. Mater. Interfaces, 11 (19). S. 17256 - 17270. WASHINGTON: AMER CHEMICAL SOC. ISSN 1944-8252

Full text not available from this repository.

Abstract

Due to the synergic feature of individual components in hybrid (nano)biomaterials, their application in regenerative medicine has drawn significant attention. Aiming to address all the current challenges of aerogel as a potent scaffold in bone tissue engineering application, we adopted a novel synthesis approach to synergistically improve the pore size regime and mechanical strength in the aerogel. The three-dimensional aerogel scaffold in this study has been synthesized through a versatile one-pot aqueous-based sol-gel hybridization/assembly of organosilane (tetraethyl orthosilicate) and silk fibroin (SF) biopolymer, followed by unidirectional freeze-casting of the as-prepared hybrid gel and supercritical drying. The developed ultralight silica-SF aerogel hybrids demonstrated a hierarchically organized porous structure with interesting honeycomb-shaped micromorphology and microstructural alignment (anisotropy) in varied length scales. The average macropore size of the hybrid aerogel lied in similar to 0.5-18 mu m and was systematically controlled with freeze-casting conditions. Together with high porosity (91-94%), high Young's modulus (similar to 4-7 MPa, >3 order of magnitude improvement compared to their pristine aerogel counterparts), and bone-type anisotropy in the mechanical compressive behavior, the silica-SF hybrid aerogel of this study acted as a very competent scaffold for bone tissue formation. The results of in vitro assessments revealed that the silica-SF aerogel is not only cytocompatible and nonhemolytic but also acted as an open porous microenvironment to trigger osteoblast cell attachment, growth, and proliferation on its surface within 14 days of incubation. Moreover, to support the in vitro results, in vivo bone formation within the aerogel implant in the bone defect site was studied. The X-ray radiology and microcomputed tomography analyses confirmed that a significant new bone tissue density formed in the defect site within 25 days of implantation. Also, in vivo toxicology studies showed a zero-toxic impact of the aerogel implant on the blood biochemical and hematological parameters. Finally, the study clearly shows the potential of aerogel as a bioactive and osteoconductive open porous cellular matrix for a successful osseointegration process.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Maleki, Hajar UNSPECIFIED orcid.org/0000-0002-2813-4700 UNSPECIFIED Shahbazi, Mohammad-Ali UNSPECIFIED orcid.org/0000-0002-4860-3017 UNSPECIFIED Montes, Susan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hosseini, Seyed Hojjat UNSPECIFIED UNSPECIFIED UNSPECIFIED Eskandari, Mohammad Reza UNSPECIFIED orcid.org/0000-0001-6219-7185 UNSPECIFIED Zaunschirm, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Verwanger, Thomas UNSPECIFIED orcid.org/0000-0003-2651-8898 UNSPECIFIED Mathur, Sanjay UNSPECIFIED orcid.org/0000-0003-2765-2693 UNSPECIFIED Milow, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Krammer, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Huesing, Nicola UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-148167
DOI:	10.1021/acsami.9b04283
Journal or Publication Title:	ACS Appl. Mater. Interfaces
Volume:	11
Number:	19
Page Range:	S. 17256 - 17270
Date:	2019
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1944-8252
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Inorganic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language THERMAL INSULATION; BIOMATERIAL SCAFFOLDS; AEROGEL HYBRIDS; TISSUE; DIFFERENTIATION; FABRICATION; COMPOSITES; POLYMERS Multiple languages Nanoscience & Nanotechnology; Materials Science, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14816