Universität zu Köln

Lange, Tobias, Walpurgis, Katja, Thomas, Andreas ORCID: 0000-0003-1199-0743, Geyer, Hans and Thevis, Mario (2019). Development of two complementary LC-HRMS methods for analyzing sotatercept in dried blood spots for doping controls. Bioanalysis, 11 (10). S. 923 - 941. LONDON: FUTURE SCI LTD. ISSN 1757-6199

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Abstract

Aim: sotatercept is a therapeutic Fc-fusion protein with erythropoiesis-stimulating activity. Due to a potential abuse of the drug by athletes in professional sports, a sensitive detection method is required. In sports drug testing, alternative matrices such as dried blood spots (DBS) are gaining increasing attention as they can provide several advantages over conventional matrices. Materials & methods: Herein, two complementary LC-high-resolution mass spectrometry (HRMS) detection methods for sotatercept from DBS, an initial testing procedure (ITP) and a confirmation procedure (CP)were developed and validated for the first time.Both methods comprise an ultrasonication-assisted extraction, affinity enrichment, proteolytic digestion and HRMS detection. Results & conclusion:For the multianalyte ITP, artificial samples fortified with sotatercept, luspatercept and bimagrumab, and authentic specimens containing bimagrumab were successfully analyzed as proof-of-concept. The validated detection methods for sotatercept are fit for purpose and the ITP was shown to be suitable for the detection of novel IgG-based pharmaceuticals in doping control DBS samples. [GRAPHICS]

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lange, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Walpurgis, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Geyer, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-148803
DOI:	10.4155/bio-2018-0313
Journal or Publication Title:	Bioanalysis
Volume:	11
Number:	10
Page Range:	S. 923 - 941
Date:	2019
Publisher:	FUTURE SCI LTD
Place of Publication:	LONDON
ISSN:	1757-6199
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FC FUSION PROTEIN; RISK MYELODYSPLASTIC SYNDROMES; GROWTH-HORMONE ABUSE; BONE-FORMATION; INCREASES HEMOGLOBIN; MAJOR METABOLITES; SYNTHETIC ANALOGS; MASS-SPECTROMETRY; INTERIM ANALYSIS; PATIENTS PTS Multiple languages Biochemical Research Methods; Chemistry, Analytical Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14880