Universität zu Köln

Nuesken, Eva, Turnwald, Eva-Maria, Fink, Gregor ORCID: 0000-0001-7042-1706, Voggel, Jenny ORCID: 0000-0001-9120-2203, Yosy, Christopher, Kretschmer, Tobias ORCID: 0000-0002-4202-0806, Handwerk, Marion, Wohlfarth, Maria, Weber, Lutz T., Hucklenbruch-Rother, Eva, Doetsch, Joerg, Nuesken, Kai-Dietrich and Appel, Sarah (2019). Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice. J. Clin. Med., 8 (5). BASEL: MDPI. ISSN 2077-0383

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Abstract

There is accumulating evidence for fetal programming of later kidney disease by maternal obesity or associated conditions. We performed a hypothesis-generating study to identify potentially underlying mechanisms. Female mice were randomly split in two groups and fed either a standard diet (SD) or high fat diet (HFD) from weaning until mating and during pregnancy. Half of the dams from both groups were treated with metformin ((M), 380 mg/kg), resulting in four experimental groups (SD, SD-M, HFD, HFD-M). Caesarean section was performed on gestational day 18.5. Fetal kidney tissue was isolated from cryo-slices using laser microdissection methods and a proteomic screen was performed. For single proteins, a fold change 1.5 and q-value <0.05 were considered to be statistically significant. Interestingly, HFD versus SD had a larger effect on the proteome of fetal kidneys (56 proteins affected; interaction clusters shown for proteins concerning transcription/translation, mitochondrial processes, eicosanoid metabolism, H2S-synthesis and membrane remodeling) than metformin exposure in either SD (29 proteins affected; clusters shown for proteins involved in transcription/translation) or HFD (6 proteins affected; no cluster). By further analysis, ATP6V1G1, THY1, PRKCA and NDUFB3 were identified as the most promising candidates potentially mediating reprogramming effects of metformin in a maternal high fat diet.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nuesken, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Turnwald, Eva-Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Gregor UNSPECIFIED orcid.org/0000-0001-7042-1706 UNSPECIFIED Voggel, Jenny UNSPECIFIED orcid.org/0000-0001-9120-2203 UNSPECIFIED Yosy, Christopher UNSPECIFIED UNSPECIFIED UNSPECIFIED Kretschmer, Tobias UNSPECIFIED orcid.org/0000-0002-4202-0806 UNSPECIFIED Handwerk, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlfarth, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Lutz T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hucklenbruch-Rother, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Nuesken, Kai-Dietrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Appel, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149062
DOI:	10.3390/jcm8050663
Journal or Publication Title:	J. Clin. Med.
Volume:	8
Number:	5
Date:	2019
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2077-0383
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOLUBLE EPOXIDE HYDROLASE; BODY-MASS INDEX; KIDNEY-DISEASE; HYDROGEN-SULFIDE; FOLLOW-UP; KINASE-C; WOMEN; HYPERTENSION; OBESITY; INHIBITION Multiple languages Medicine, General & Internal Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14906