Universität zu Köln

Rumpel, Sonja, Scholl, Christina, Goebel, Anja, Palme, Rupert ORCID: 0000-0001-9466-3662 and Mahabir, Esther (2019). Effect of Ground Transportation on Adrenocortical Activity in Prepuberal Female Mice from Five Different Genetic Backgrounds. Animals, 9 (5). BASEL: MDPI. ISSN 2076-2615

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Abstract

Simple Summary For research purposes, mice are often transported between institutions, which may elicit stress, thereby influencing results. We determined adrenocortical activity by measuring fecal corticosterone metabolites (FCMs), as a stress marker, in prepuberal mice from five genetic backgrounds, namely C57BL/6J, C57BL/6NCrl, FVB/NCrl, Crl:CD1(ICR), and BALB/cAnCrl. Only C57BL/6N showed significantly higher FCM levels the day after transport, but baseline levels were attained within four days. Abstract Specific experimental protocols necessitate transportation, a potentially stressful event that could confound results. We determined adrenocortical activity by measuring fecal corticosterone metabolites (FCMs), as a stress marker, in prepuberal (three-week old) female C57BL/6J, C57BL/6NCrl, FVB/NCrl, Crl:CD1(ICR), and BALB/cAnCrl mice. On each transport day, five female cage mates per genetic background were weaned and transported in stable groups via truck from the breeding to the research facility. Fecal pellets were collected on Days 0, 1, and 4. Mice were superovulated for embryo production to determine if repeated fecal collection impacts this procedure. The average duration of transportation over 600 km and from packing to unpacking of mice was 7.24 and 22.62 h, respectively. FCM levels increased from Day 0 to Day 1 and decreased on Day 4 in all genetic backgrounds except in FVB/NCrl, but only B6N showed significantly higher FCM levels on Day 1. Furthermore, embryo production was not affected by repeated feces collection. The results show that weaning and immediate transport of prepuberal mice from the breeding to the research facility led to temporal and genetic background-dependent increases of adrenocortical activity in four of the five genetic backgrounds investigated, which returned to baseline levels within four days.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rumpel, Sonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholl, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Goebel, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Palme, Rupert UNSPECIFIED orcid.org/0000-0001-9466-3662 UNSPECIFIED Mahabir, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149080
DOI:	10.3390/ani9050239
Journal or Publication Title:	Animals
Volume:	9
Number:	5
Date:	2019
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-2615
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BODY-WEIGHT; STRESS; CORTICOSTERONE; METABOLITES; INDICATORS Multiple languages Agriculture, Dairy & Animal Science; Veterinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14908