Universität zu Köln

Konopova, Barbora ORCID: 0000-0002-8456-9530, Kolosov, Dennis and O'Donnell, Michael J. (2019). Water and ion transport across the eversible vesicles in the collophore of the springtail Orchesella cincta. J. Exp. Biol., 222 (10). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9145

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Abstract

Springtails (Collembola) are ancient close relatives of the insects. The eversible vesicles are their unique paired transporting organs, which consist of an epithelium located inside a tube-like structure called the collophore on the first abdominal segment. The vesicles can be protruded out of the collophore and several lines of evidence indicate that they have a vital function in water uptake and ion balance. However, the amount of water absorbed by the vesicles and which other ions apart from Na+ are transported remain unknown. Using Orchesella cincta as a model, we developed protocols for two assays that enabled us to study water and ion movement across the eversible vesicles in whole living springtails. Using an inverse Ramsay assay we demonstrate that the eversible vesicles absorb water from a droplet applied onto their surface. Using the scanning ion-selective electrode technique (SIET), we show that the vesicles absorb Na+ and Cl- from the bathing medium, secrete NH4+, and both absorb and secrete K+. H+ is secreted at a low level in the anterior part and absorbed at the posterior part. We did not detect transport of Ca2+ at significant levels. The highest flux was the absorption of Cl-, and the magnitude of ion fluxes was significantly lower in fully hydrated springtails. Our data demonstrate that the eversible vesicles are a transporting epithelium functioning in osmo-and ionoregulation, nitrogenous waste excretion and probably also acid-base balance.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Konopova, Barbora UNSPECIFIED orcid.org/0000-0002-8456-9530 UNSPECIFIED Kolosov, Dennis UNSPECIFIED UNSPECIFIED UNSPECIFIED O'Donnell, Michael J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149293
DOI:	10.1242/jeb.200691
Journal or Publication Title:	J. Exp. Biol.
Volume:	222
Number:	10
Date:	2019
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9145
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TOMOCERUS-FLAVESCENS TULLBERG; AQUATICA L. COLLEMBOLA; VENTRAL TUBE; MALPIGHIAN TUBULES; DROSOPHILA-MELANOGASTER; CRYOPROTECTIVE DEHYDRATION; VAPOR ABSORPTION; K+ TRANSPORT; 1871 INSECTA; EPITHELIUM Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14929