Universität zu Köln

Wawra, Stephan ORCID: 0000-0001-8555-1618, Fesel, Philipp, Widmer, Heidi, Neumann, Ulla, Lahrmann, Urs, Becker, Stefan ORCID: 0000-0002-9619-3542, Hehemann, Jan-Hendrik ORCID: 0000-0002-8700-2564, Langen, Gregor ORCID: 0000-0002-8321-1756 and Zuccaro, Alga ORCID: 0000-0002-8026-0114 (2019). FGB1 and WSC3 are in planta-induced beta-glucan-binding fungal lectins with different functions. New Phytol., 222 (3). S. 1493 - 1507. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

In the root endophyte Serendipita indica, several lectin-like members of the expanded multigene family of WSC proteins are transcriptionally induced in planta and are potentially involved in beta-glucan remodeling at the fungal cell wall. Using biochemical and cytological approaches we show that one of these lectins, SiWSC3 with three WSC domains, is an integral fungal cell wall component that binds to long-chain beta 1-3-glucan but has no affinity for shorter beta 1-3- or beta 1-6-linked glucose oligomers. Comparative analysis with the previously identified beta-glucan-binding lectin SiFGB1 demonstrated that whereas SiWSC3 does not require beta 1-6-linked glucose for efficient binding to branched beta 1-3-glucan, SiFGB1 does. In contrast to SiFGB1, the multivalent SiWSC3 lectin can efficiently agglutinate fungal cells and is additionally induced during fungus-fungus confrontation, suggesting different functions for these two beta-glucan-binding lectins. Our results highlight the importance of the beta-glucan cell wall component in plant-fungus interactions and the potential of beta-glucan-binding lectins as specific detection tools for fungi in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wawra, Stephan UNSPECIFIED orcid.org/0000-0001-8555-1618 UNSPECIFIED Fesel, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Widmer, Heidi UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumann, Ulla UNSPECIFIED UNSPECIFIED UNSPECIFIED Lahrmann, Urs UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Stefan UNSPECIFIED orcid.org/0000-0002-9619-3542 UNSPECIFIED Hehemann, Jan-Hendrik UNSPECIFIED orcid.org/0000-0002-8700-2564 UNSPECIFIED Langen, Gregor UNSPECIFIED orcid.org/0000-0002-8321-1756 UNSPECIFIED Zuccaro, Alga UNSPECIFIED orcid.org/0000-0002-8026-0114 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149818
DOI:	10.1111/nph.15711
Journal or Publication Title:	New Phytol.
Volume:	222
Number:	3
Page Range:	S. 1493 - 1507
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-WALL INTEGRITY; CHITIN-TRIGGERED IMMUNITY; STRESS-RESPONSE; PROTEIN; GENES; SUPPRESSION; INFECTION; EVASION; DOMAIN; LOCALIZATION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14981