Universität zu Köln

Wang, Duo, Wang, Yumei, Liu, Huan, Tong, Chang, Ying, Qilong, Sachinidis, Agapios, Li, Li and Peng, Luying (2019). Laminin promotes differentiation of rat embryonic stem cells into cardiomyocytes by activating the integrin/FAK/PI3K p85 pathway. J. Cell. Mol. Med., 23 (5). S. 3629 - 3641. HOBOKEN: WILEY. ISSN 1582-4934

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Abstract

The generation of germline competent rat embryonic stem cells (rESCs) allows the study of their lineage commitment. Here, we developed a highly efficient system for rESC-derived cardiomyocytes, and even the formation of three-dimensional (3D)-like cell clusters with cTNT and alpha-Actinin. We have validated that laminin can interact with membrane integrin to promote the phosphorylation of both phosphatidylinositol 3-kinase (PI3K) p85 and the focal adhesion kinase (FAK). In parallel, GATA4 was up-regulated. Upon inhibiting the integrin, laminin loses the effect on cardiomyocyte differentiation, accompanied with a down-regulation of phosphorylation level of PI3K p85 and FAK. Meanwhile, the expression of Gata4 was inhibited as well. Taken together, laminin is a crucial component in the differentiation of rESCs into cardiomyocytes through increasing their proliferation via interacting with integrin pathway. These results provide new insights into the pathways mediated by extracellular laminin involved in the fate of rESC-derived cardiomyocytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Duo UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Yumei UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Huan UNSPECIFIED UNSPECIFIED UNSPECIFIED Tong, Chang UNSPECIFIED UNSPECIFIED UNSPECIFIED Ying, Qilong UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Peng, Luying UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149849
DOI:	10.1111/jcmm.14264
Journal or Publication Title:	J. Cell. Mol. Med.
Volume:	23
Number:	5
Page Range:	S. 3629 - 3641
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1582-4934
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENE KNOCKOUT RATS; EXTRACELLULAR-MATRIX; HOMOLOGOUS RECOMBINATION; CARDIAC DIFFERENTIATION; IN-VITRO; MOUSE; ADHESION; SIGNALS; BMP Multiple languages Cell Biology; Medicine, Research & Experimental Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14984