Universität zu Köln

Anoman, Armand D., Flores-Tornero, Maria ORCID: 0000-0002-9296-0070, Benstein, Ruben M., Blau, Samira, Rosa-Tellez, Sara, Braeutigam, Andrea, Fernie, Alisdair R., Munoz-Bertomeu, Jesus ORCID: 0000-0002-2099-3754, Schilasky, Soeren, Meyer, Andreas J., Kopriva, Stanislav ORCID: 0000-0002-7416-6551, Segura, Juan ORCID: 0000-0001-7774-2676, Krueger, Stephan and Ros, Roc ORCID: 0000-0003-4254-8368 (2019). Deficiency in the Phosphorylated Pathway of Serine Biosynthesis Perturbs Sulfur Assimilation. Plant Physiol., 180 (1). S. 153 - 171. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

Although the plant Phosphorylated Pathway of L-Ser Biosynthesis (PPSB) is essential for embryo and pollen development, and for root growth, its metabolic implications have not been fully investigated. A transcriptomics analysis of Arabidopsis (Arabidopsis thaliana) PPSB-deficient mutants at night, when PPSB activity is thought to be more important, suggested interaction with the sulfate assimilation process. Because sulfate assimilation occurs mainly in the light, we also investigated it in PPSB-deficient lines in the day. Key genes in the sulfate starvation response, such as the adenosine 5'phosphosulfate reductase genes, along with sulfate transporters, especially those involved in sulfate translocation in the plant, were induced in the PPSB-deficient lines. However, sulfate content was not reduced in these lines as compared with wild-type plants; besides the glutathione (GSH) steady-state levels in roots of PPSB-deficient lines were even higher than in wild type. This suggested that PPSB deficiency perturbs the sulfate assimilation process between tissues/organs. Alteration of thiol distribution in leaves from different developmental stages, and between aerial parts and roots in plants with reduced PPSB activity, provided evidence supporting this idea. Diminished PPSB activity caused an enhanced flux of S-35 into thiol biosynthesis, especially in roots. GSH turnover also accelerated in the PPSB-deficient lines, supporting the notion that not only biosynthesis, but also transport and allocation, of thiols were perturbed in the PPSB mutants. Our results suggest that PPSB is required for sulfide assimilation in specific heterotrophic tissues and that a lack of PPSB activity perturbs sulfur homeostasis between photosynthetic and nonphotosynthetic tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Anoman, Armand D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Flores-Tornero, Maria UNSPECIFIED orcid.org/0000-0002-9296-0070 UNSPECIFIED Benstein, Ruben M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Blau, Samira UNSPECIFIED UNSPECIFIED UNSPECIFIED Rosa-Tellez, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Braeutigam, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Munoz-Bertomeu, Jesus UNSPECIFIED orcid.org/0000-0002-2099-3754 UNSPECIFIED Schilasky, Soeren UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Andreas J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Segura, Juan UNSPECIFIED orcid.org/0000-0001-7774-2676 UNSPECIFIED Krueger, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Ros, Roc UNSPECIFIED orcid.org/0000-0003-4254-8368 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149852
DOI:	10.1104/pp.18.01549
Journal or Publication Title:	Plant Physiol.
Volume:	180
Number:	1
Page Range:	S. 153 - 171
Date:	2019
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FALSE DISCOVERY RATE; SULFATE ASSIMILATION; 3-PHOSPHOGLYCERATE DEHYDROGENASE; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; AMINO-ACID; MOLECULAR CHARACTERIZATION; GLUTATHIONE-REDUCTASE; GAS-CHROMATOGRAPHY; ATP SULFURYLASE Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14985