Universität zu Köln

Villamil, Johana C. Misas, Mueller, Andre N., Demir, Fatih ORCID: 0000-0002-5744-0205, Meyer, Ute, Oekmen, Bilal, Hueynck, Jan Schulze, Breuer, Marlen, Dauben, Helen, Win, Joe ORCID: 0000-0002-9851-2404, Huesgen, Pitter F. and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2019). A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif. Nat. Commun., 10. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Ustilago maydis is a biotrophic fungus causing corn smut disease in maize. The secreted effector protein Pit2 is an inhibitor of papain-like cysteine proteases (PLCPs) essential for virulence. Pit2 inhibitory function relies on a conserved 14 amino acids motif (PID14). Here we show that synthetic PID14 peptides act more efficiently as PLCP inhibitors than the full-length Pit2 effector. Mass spectrometry shows processing of Pit2 by maize PLCPs, which releases an inhibitory core motif from the PID14 sequence. Mutational analysis demonstrates that two conserved residues are essential for Pit2 function. We propose that the Pit2 effector functions as a substrate mimicking molecule: Pit2 is a suitable substrate for apoplastic PLCPs and its processing releases the embedded inhibitor peptide, which in turn blocks PLCPs to modulate host immunity. Remarkably, the PID14 core motif is present in several plant associated fungi and bacteria, indicating the existence of a conserved microbial inhibitor of proteases (cMIP).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Villamil, Johana C. Misas UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Andre N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Demir, Fatih UNSPECIFIED orcid.org/0000-0002-5744-0205 UNSPECIFIED Meyer, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Oekmen, Bilal UNSPECIFIED UNSPECIFIED UNSPECIFIED Hueynck, Jan Schulze UNSPECIFIED UNSPECIFIED UNSPECIFIED Breuer, Marlen UNSPECIFIED UNSPECIFIED UNSPECIFIED Dauben, Helen UNSPECIFIED UNSPECIFIED UNSPECIFIED Win, Joe UNSPECIFIED orcid.org/0000-0002-9851-2404 UNSPECIFIED Huesgen, Pitter F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-150984
DOI:	10.1038/s41467-019-09472-8
Journal or Publication Title:	Nat. Commun.
Volume:	10
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language USTILAGO-MAYDIS; CYSTEINE PROTEASES; PEPTIDE LIBRARIES; SECRETED EFFECTOR; SALICYLIC-ACID; PROTEIN; CLEAVAGE; GENES; IDENTIFICATION; VIRULENCE Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15098