Schultheis, Dorothea, Weisskopf, Matthias, Schaub, Christoph ORCID: 0000-0003-3714-0050, Ansari, Salim, Grossmann, Daniela, Majumdar, Upalparna, Hakeemi, Muhammad Salim, Troelenberg, Nicole, Richter, Tobias, Schmitt-Engel, Christian, Schwirz, Jonas, Stroehlein, Nadi, Teuscher, Matthias ORCID: 0000-0003-2340-5716, Bucher, Gregor and Frasch, Manfred (2019). A Large Scale Systemic RNAi Screen in the Red Flour Beetle Tribolium castaneum Identifies Novel Genes Involved in Insect Muscle Development. G3-Genes Genomes Genet., 9 (4). S. 1009 - 1027. BETHESDA: GENETICS SOCIETY AMERICA. ISSN 2160-1836

Full text not available from this repository.

Abstract

Although muscle development has been widely studied in Drosophila melanogaster there are still many gaps in our knowledge, and it is not known to which extent this knowledge can be transferred to other insects. To help in closing these gaps we participated in a large-scale RNAi screen that used the red flour beetle, Tribolium castaneum, as a screening platform. The effects of systemic RNAi were screened upon double-stranded RNA injections into appropriate muscle-EGFP tester strains. Injections into pupae were followed by the analysis of the late embryonic/early larval muscle patterns, and injections into larvae by the analysis of the adult thoracic muscle patterns. Herein we describe the results of the first-pass screens with pupal and larval injections, which covered similar to 8,500 and similar to 5,000 genes, respectively, of a total of similar to 16,500 genes of the Tribolium genome. Apart from many genes known from Drosophila as regulators of muscle development, a collection of genes previously unconnected to muscle development yielded phenotypes in larval body wall and leg muscles as well as in indirect flight muscles. We then present the main candidates from the pupal injection screen that remained after being processed through a series of verification and selection steps. Further, we discuss why distinct though overlapping sets of genes are revealed by the Drosophila and Tribolium screening approaches.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Schultheis, DorotheaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weisskopf, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaub, ChristophUNSPECIFIEDorcid.org/0000-0003-3714-0050UNSPECIFIED
Ansari, SalimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grossmann, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Majumdar, UpalparnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hakeemi, Muhammad SalimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Troelenberg, NicoleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Richter, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schmitt-Engel, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schwirz, JonasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stroehlein, NadiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Teuscher, MatthiasUNSPECIFIEDorcid.org/0000-0003-2340-5716UNSPECIFIED
Bucher, GregorUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Frasch, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-152053
DOI: 10.1534/g3.118.200995
Journal or Publication Title: G3-Genes Genomes Genet.
Volume: 9
Number: 4
Page Range: S. 1009 - 1027
Date: 2019
Publisher: GENETICS SOCIETY AMERICA
Place of Publication: BETHESDA
ISSN: 2160-1836
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TRANSCRIPTION FACTOR; MYOBLAST FUSION; SHORT-GERM; SOMATIC MUSCULATURE; MYOTUBE GUIDANCE; CELL FATES; DROSOPHILA; PROTEIN; EXPRESSION; REGULATORMultiple languages
Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15205

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item