Universität zu Köln

Nguyen, Dustin M., Wagenhauser, Markus U., Mehrkens, Dennis ORCID: 0000-0002-8578-0290, Adam, Matti ORCID: 0000-0002-6990-8135, Tsao, Philip S. and Ramasubramanian, Anand K. (2019). An Automated Algorithm to Quantify Collagen Distribution in Aortic Wall. J. Histochem. Cytochem., 67 (4). S. 267 - 275. LONDON: SAGE PUBLICATIONS LTD. ISSN 1551-5044

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Abstract

Arterial diseases including abdominal aortic aneurysm and atherosclerosis are biomechanical diseases characterized by significant changes in the structure and strength of the vessel wall. It is now established that local variations in fibrillar collagen and elastin matrix turnover is critical to arterial stiffening and progression of the disease. The collagen content in the aortic wall has nominally been quantified by biochemical assays and immunohistochemical analysis as the total amount because of the difficulty in separating the media and adventitia. In this work, we have developed an algorithm for automatic quantification of layer-specific collagen content from bright-field and polarized microscopic images of histological sections of mouse aorta stained with Picrosirius red (PSR) stain. The images were processed sequentially including separation of layers, erosion, segregation of regions, binarization, and quantification of pixel intensities to obtain collagen content in the media and adventitia separately. We observed that the automated algorithm rapidly and accurately quantified collagen content from a wide range of image quality compared with manual measurements particularly when the medial and adventitial layers overlap. Together, our algorithm will be of significant impact in the rapid, reliable, and accurate analyses of collagen distribution in histological sections of connective tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nguyen, Dustin M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagenhauser, Markus U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mehrkens, Dennis UNSPECIFIED orcid.org/0000-0002-8578-0290 UNSPECIFIED Adam, Matti UNSPECIFIED orcid.org/0000-0002-6990-8135 UNSPECIFIED Tsao, Philip S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ramasubramanian, Anand K. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-152828
DOI:	10.1369/0022155418814231
Journal or Publication Title:	J. Histochem. Cytochem.
Volume:	67
Number:	4
Page Range:	S. 267 - 275
Date:	2019
Publisher:	SAGE PUBLICATIONS LTD
Place of Publication:	LONDON
ISSN:	1551-5044
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FLUORESCENCE Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15282