Universität zu Köln

Bunte, Sebastian, Lill, Tobias, Falk, Maximilian, Stroethoff, Martin, Raupach, Annika, Mathes, Alexander, Heinen, Andre, Hollmann, Markus W. and Huhn, Ragnar (2019). Impact of Anesthetics on Cardioprotection Induced by Pharmacological Preconditioning. J. Clin. Med., 8 (3). BASEL: MDPI. ISSN 2077-0383

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Abstract

Anesthetics, especially propofol, are discussed to influence ischemic preconditioning. We investigated whether cardioprotection by milrinone or levosimendan is influenced by the clinically used anesthetics propofol, sevoflurane or dexmedetomidine. Hearts of male Wistar rats were randomised, placed on a Langendorff system and perfused with Krebs-Henseleit buffer (KHB) at a constant pressure of 80 mmHg. All hearts underwent 33 min of global ischemia and 60 min of reperfusion. Three different anesthetic regimens were conducted throughout the experiments: propofol (11 mu M), sevoflurane (2.5 Vol%) and dexmedetomidine (1.5 nM). Under each anesthetic regimen, pharmacological preconditioning was induced by administration of milrinone (1 mu M) or levosimendan (0.3 mu M) 10 min before ischemia. Infarct size was determined by TTC staining. Infarct sizes in control groups were comparable (KHB-Con: 53 +/- 9%, Prop-Con: 56 +/- 9%, Sevo-Con: 56 +/- 8%, Dex-Con: 53 +/- 9%; ns). Propofol completely abolished preconditioning by milrinone and levosimendan (Prop-Mil: 52 +/- 8%, Prop-Lev: 52 +/- 8%; ns versus Prop-Con), while sevoflurane did not (Sevo-Mil: 31 +/- 9%, Sevo-Lev: 33 +/- 7%; p < 0.05 versus Sevo-Con). Under dexmedetomidine, results were inconsistent; levosimendan induced infarct size reduction (Dex-Lev: 36 +/- 6%; p < 0.05 versus Dex-Con) but not milrinone (Dex-Mil: 51 +/- 8%; ns versus Dex-Con). The choice of the anesthetic regimen has an impact on infarct size reduction by pharmacological preconditioning.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bunte, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lill, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Falk, Maximilian UNSPECIFIED UNSPECIFIED UNSPECIFIED Stroethoff, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Raupach, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathes, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinen, Andre UNSPECIFIED UNSPECIFIED UNSPECIFIED Hollmann, Markus W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huhn, Ragnar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-153478
DOI:	10.3390/jcm8030396
Journal or Publication Title:	J. Clin. Med.
Volume:	8
Number:	3
Date:	2019
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2077-0383
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REMOTE; SEVOFLURANE; ISCHEMIA; SURGERY; INJURY; BYPASS; LEVOSIMENDAN; MYOCARDIUM; ACTIVATION; PROTECTION Multiple languages Medicine, General & Internal Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15347