Universität zu Köln

Dennenmoser, Stefan, Sedlazeck, Fritz J., Schatz, Michael C., Altmueller, Janine, Zytnicki, Matthias and Nolte, Arne W. (2019). Genome-wide patterns of transposon proliferation in an evolutionary young hybrid fish. Mol. Ecol., 28 (6). S. 1491 - 1506. HOBOKEN: WILEY. ISSN 1365-294X

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Abstract

Hybridization can induce transposons to jump into new genomic positions, which may result in their accumulation across the genome. Alternatively, transposon copy numbers may increase through nonallelic (ectopic) homologous recombination in highly repetitive regions of the genome. The relative contribution of transposition bursts versus recombination-based mechanisms to evolutionary processes remains unclear because studies on transposon dynamics in natural systems are rare. We assessed the genomewide distribution of transposon insertions in a young hybrid lineage (invasive Cottus, n = 11) and its parental species Cottus rhenanus (n = 17) and Cottus perifretum(n = 9) using a reference genome assembled from long single molecule pacbio reads. An inventory of transposable elements was reconstructed from the same data and annotated. Transposon copy numbers in the hybrid lineage increased in 120 (15.9%) out of 757 transposons studied here. The copy number increased on average by 69% (range: 10%-197%). Given the age of the hybrid lineage, this suggests that they have proliferated within a few hundred generations since admixture began. However, frequency spectra of transposon insertions revealed no increase in novel and rare insertions across assembled parts of the genome. This implies that transposons were added to repetitive regions of the genome that remain difficult to assemble. Future studies will need to evaluate whether recombination-based mechanisms rather than genomewide transposition may explain the majority of the recent transposon proliferation in the hybrid lineage. Irrespectively of the underlying mechanism, the observed overabundance in repetitive parts of the genome suggests that gene-rich regions are unlikely to be directly affected.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dennenmoser, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Sedlazeck, Fritz J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schatz, Michael C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Zytnicki, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Nolte, Arne W. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-154668
DOI:	10.1111/mec.14969
Journal or Publication Title:	Mol. Ecol.
Volume:	28
Number:	6
Page Range:	S. 1491 - 1506
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-294X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NON-LTR RETROTRANSPOSON; POPULATION-DYNAMICS; ELEMENT; COTTUS; TELEOSTEI; PISCES; ORGANIZATION; CHROMOSOMES; INSTABILITY; CENTROMERES Multiple languages Biochemistry & Molecular Biology; Ecology; Evolutionary Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15466