Universität zu Köln

Koloren, Onur, Koloren, Zeynep, Sekeroglu, Zuelal Atli, Colayvaz, Melek and Karanis, Panagiotis (2019). Amoebicidal and Amoebistatic Effects of Artemisia argyi Methanolic Extracts on Acanthamoeba castellanii Trophozoites and Cysts. Acta Parasitolog., 64 (1). S. 63 - 71. CHAM: SPRINGER INTERNATIONAL PUBLISHING AG. ISSN 1896-1851

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Abstract

PurposeThe present study aimed to investigate the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and on cysts. We also determined cytotoxic effect, enzymatic and non-enzymatic antioxidant activities, total phenolic, lavonoid and antioxidative contents of A. argyi.MethodsA. argyi was harvested from various geographic sites in Ordu province in Turkey. The fresh leaves were subjected to methanolic extraction. In 100 l culture, different concentrations of A. argyi methanolic extract (in quantities from 1.2, 2.3, 4.7, 9.4, 18.7, 37.4, 74.8 mg/ml) and the same volume of trophozoite/cyst suspension were mixed for the determination of the amoebicidal activity of the plant extract. Human bronchial epithelial cells were treated with the same concentrations of Artemisia extracts to determine cytotoxic potential.ResultsTotal phenolic and lavonoid contents of the extract were calculated as 261 mg gallic acid/g dry extract and 29 mg quercetin/g dry extract, respectively. Total antioxidant activity was also calculated as 367 mg ascorbic acid/g dry extract. The growth of trophozoites stopped in A. argyi methanolic extract with 50% inhibitory concentrations (IC50)/8 h for 37.4 mg/ ml and 74.8 mg/ml extract solution and had stronger amoebicidal activity on the cysts with IC50/72 h. Artemisia showed stronger inhibitory effects on bronchial epithelial cells at the concentrations of 9.4, 18.7, 37.4 and 74.8 mg/ml.ConclusionThe study indicated that A. argyi leaf extract has cytotoxic and anti-amoebic activities.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koloren, Onur UNSPECIFIED UNSPECIFIED UNSPECIFIED Koloren, Zeynep UNSPECIFIED UNSPECIFIED UNSPECIFIED Sekeroglu, Zuelal Atli UNSPECIFIED UNSPECIFIED UNSPECIFIED Colayvaz, Melek UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-154843
DOI:	10.2478/s11686-018-00009-5
Journal or Publication Title:	Acta Parasitolog.
Volume:	64
Number:	1
Page Range:	S. 63 - 71
Date:	2019
Publisher:	SPRINGER INTERNATIONAL PUBLISHING AG
Place of Publication:	CHAM
ISSN:	1896-1851
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHEMICAL-CONSTITUENTS; ANTIOXIDANT ACTIVITY; L.; KERATITIS; LEAF; IDENTIFICATION; ABILITY; ASSAY Multiple languages Parasitology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15484