Universität zu Köln

Dietze-Schwonberg, Kirsten, Kostka, Susanna Lopez, Lorenz, Beate, Regen, Tommy, Waisman, Ari and von Stebut, Esther (2019). Proteomic analysis of stratum corneum in Cutaneous T-Cell Lymphomas and psoriasis. Exp. Dermatol., 28 (3). S. 317 - 324. HOBOKEN: WILEY. ISSN 1600-0625

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Abstract

Stratum corneum collected by tape stripping from 10 and 24 subjects with cutaneous T-cell lymphomas (CTCL) or psoriasis, respectively, were compared using quantitative label-free mass spectrometry analysis. A non-supervised statistical analysis (Posneg NMF) based on 352 differentially expressed proteins in both CTCL and psoriasis samples was able to separate the two disease groups and finally able to identify a set of 112 proteins that contributed most and significantly to the separation when compared to non-lesional samples. In addition, Luminex assay revealed that the increase in the amount of chemokines related to the inflammatory response, and immune cell infiltration and recruitment in lesional stratum corneum in CTCL, including CXCL8, CXCL9, CXCL10, CCL27, TNF and sICAM-1 was in agreement with published data on entire skin biopsies. Proteome analysis using quantitative methods including mass spectrometry and Luminex technology offered the possibility to investigate the relevant protein signature in CTCL and may be helpful to diagnose and investigate the efficacy of treatments in clinical investigations using non-invasive methods in future.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dietze-Schwonberg, Kirsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Kostka, Susanna Lopez UNSPECIFIED UNSPECIFIED UNSPECIFIED Lorenz, Beate UNSPECIFIED UNSPECIFIED UNSPECIFIED Regen, Tommy UNSPECIFIED UNSPECIFIED UNSPECIFIED Waisman, Ari UNSPECIFIED UNSPECIFIED UNSPECIFIED von Stebut, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-155297
DOI:	10.1111/exd.13880
Journal or Publication Title:	Exp. Dermatol.
Volume:	28
Number:	3
Page Range:	S. 317 - 324
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1600-0625
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ELEVATED SERUM CTACK/CCL27; EXPRESSION; IP-10; GAMMA Multiple languages Dermatology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15529