Universität zu Köln

Iacobucci, Claudio ORCID: 0000-0001-9592-3606, Schaefer, Mathias ORCID: 0000-0002-5943-4335 and Sinz, Andrea (2019). Free radical-initiated peptide sequencing (FRIPS)-based cross-linkers for improved peptide and protein structure analysis. Mass Spectrom. Rev., 38 (2). S. 187 - 202. HOBOKEN: WILEY. ISSN 1098-2787

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Abstract

Free radical-initiated peptide sequencing (FRIPS) has recently been introduced as an analytical strategy to create peptide radical ions in a predictable and effective way by collisional activation of specifically modified peptides ions. FRIPS is based on the unimolecular dissociation of open-shell ions and yields fragments that resemble those obtained by electron capture dissociation (ECD) or electron transfer dissociation (ETD). In this review article, we describe the fundamentals of FRIPS and highlight its fruitful combination with chemical cross-linking/mass spectrometry (MS) as a highly promising option to derive complementary structural information of peptides and proteins. FRIPS does not only yield exhaustive sequence information of cross-linked peptides, but also defines the exact cross-linking sites of the connected peptides. The development of more advanced FRIPS cross-linkers that extend the FRIPS-based cross-linking/MS approach to the study of large protein assemblies and protein interaction networks can be eagerly anticipated.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Iacobucci, Claudio UNSPECIFIED orcid.org/0000-0001-9592-3606 UNSPECIFIED Schaefer, Mathias UNSPECIFIED orcid.org/0000-0002-5943-4335 UNSPECIFIED Sinz, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-156098
DOI:	10.1002/mas.21568
Journal or Publication Title:	Mass Spectrom. Rev.
Volume:	38
Number:	2
Page Range:	S. 187 - 202
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1098-2787
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Organic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ELECTRON-CAPTURE DISSOCIATION; COLLISION-INDUCED DISSOCIATION; INFRARED MULTIPHOTON DISSOCIATION; DISULFIDE BOND-CLEAVAGE; MASS-SPECTROMETRY; GAS-PHASE; AMINO-ACID; DIRECTED DISSOCIATION; ELECTROMAGNETIC TRAPS; AMBIENT IONIZATION Multiple languages Spectroscopy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15609