Universität zu Köln

Klotz, Peter, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Schaubmar, Andreas R., Failing, Klaus, Leidner, Ursula, Seifert, Harald, Scheufen, Sandra, Semmler, Torsten ORCID: 0000-0002-2225-7267 and Ewers, Christa (2019). Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany. Front. Microbiol., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

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Abstract

Acinetobacter baumannii is one of the leading causes of nosocomial infections in humans. To investigate its prevalence, distribution of sequence types (STs), and antimicrobial resistance in cattle, we sampled 422 cattle, including 280 dairy cows, 59 beef cattle, and 83 calves over a 14-month period. Metadata, such as the previous use of antimicrobial agents and feeding, were collected to identify putative determining factors. Bacterial isolates were identified via MALDI-TOF/MS and PCR, antimicrobial susceptibility was evaluated via VITEK2 and antibiotic gradient tests, resistance genes were identified by PCR. Overall, 15.6% of the cattle harbored A. baumannii, predominantly in the nose (60.3% of the A. baumannii isolates). It was more frequent in dairy cows (21.1%) than in beef cattle (6.8%) and calves (2.4%). A seasonal occurrence was shown with a peak between May and August. The rate of occurrence of A. baumannii was correlated with a history of use of 3rd generation cephalosporins in the last 6 months prior to sampling Multilocus sequence typing (Pasteur scheme) revealed 83 STs among 126 unique isolates. Nine of the bovine STs have previously been implicated in human infections. Besides known intrinsic resistance of the species, the isolates did not show additional resistance to the antimicrobial substances tested, including carbapenems. Our data suggest that cattle are not a reservoir for nosocomial A. baumannii but carry a highly diverse population of this species. Nevertheless, some STs seem to be able to colonize both cattle and humans.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klotz, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Schaubmar, Andreas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Failing, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Leidner, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheufen, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Semmler, Torsten UNSPECIFIED orcid.org/0000-0002-2225-7267 UNSPECIFIED Ewers, Christa UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-156340
DOI:	10.3389/fmicb.2019.00272
Journal or Publication Title:	Front. Microbiol.
Volume:	10
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NOSOCOMIAL PATHOGEN; ANTIMICROBIAL SUSCEPTIBILITY; MOLECULAR CHARACTERIZATION; ANTIBIOTIC-RESISTANCE; VETERINARY CLINICS; REUNION ISLAND; MULTIPLEX PCR; SEQUENCE; BACTERIA; SPREAD Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15634