Universität zu Köln

Kieliba, Tobias ORCID: 0000-0002-9238-5336, Lerch, Oliver, Andresen-Streichert, Hilke, Rothschild, Markus A. and Beike, Justus (2019). Simultaneous quantification of THC-COOH, OH-THC, and further cannabinoids in human hair by gas chromatography-tandem mass spectrometry with electron ionization applying automated sample preparation. Drug Test. Anal., 11 (2). S. 267 - 279. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

The detection of Delta(9)-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair, for the purpose of identifying cannabis consumption, is conducted in many forensic laboratories. Since external contamination of hair with these cannabis components cannot be excluded, even after hair decontamination, only the detection of THC metabolites such as 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH) or 11-hydroxy-Delta(9)-tetrahydrocannabinol (OH-THC), is considered to prove cannabis consumption. At present, testing for THC metabolites is not standard practice due to its analytical complexity. For these reasons, we developed a novel method for the detection of THC-COOH and OH-THC as well as THC, CBD, and CBN in one single analytical run using gas chromatography-tandem mass spectrometry (GC-MS/MS) with electron ionization. After manual hair washing and grinding, sample preparation was fully automated, by means of a robotic autosampler. The hair extraction took place by digestion with sodium hydroxide. A solid-phase extraction (SPE) was chosen for sample clean-up, using a mixed-mode anion exchange sorbent. Derivatization of all analytes was by silylation. The method has been fully validated according to guidelines of the Society of Toxicological and Forensic Chemistry (GTFCh), with a limit of detection (LOD) of 0.2 pg/mg for THC-COOH and OH-THC and 2 pg/mg for THC, CBD and CBN, respectively, thus fulfilling the Society of Hair Testing (SoHT) recommendations. The validated method has been successfully applied to our routine forensic case work and a summary of data from authentic hair samples is given, as well as data from proficiency tests.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kieliba, Tobias UNSPECIFIED orcid.org/0000-0002-9238-5336 UNSPECIFIED Lerch, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Andresen-Streichert, Hilke UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothschild, Markus A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Beike, Justus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-157542
DOI:	10.1002/dta.2490
Journal or Publication Title:	Drug Test. Anal.
Volume:	11
Number:	2
Page Range:	S. 267 - 279
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOLID-PHASE MICROEXTRACTION; TESTING HUMAN HAIR; 11-NOR-DELTA(9)-TETRAHYDROCANNABINOL-9-CARBOXYLIC ACID; 11-NOR-DELTA-9-TETRAHYDROCANNABINOL-9-CARBOXYLIC ACID; DYNAMIC EXTRACTION; DRUG INCORPORATION; WORKPLACE DRUG; DELTA(9)-TETRAHYDROCANNABINOL; IDENTIFICATION; CANNABIDIOL Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15754