Universität zu Köln

Lippert, Rachel N., Cremer, Anna Lena, Thanarajah, Sharmili Edwin, Korn, Clio, Jahans-Price, Thomas, Burgeno, Lauren M., Tittgemeyer, Marc, Bruening, Jens C., Walton, Mark E. and Backes, Heiko (2019). Time-dependent assessment of stimulus-evoked regional dopamine release. Nat. Commun., 10. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

To date, the spatiotemporal release of specific neurotransmitters at physiological levels in the human brain cannot be detected. Here, we present a method that relates minute-by-minute fluctuations of the positron emission tomography (PET) radioligand [11C]raclopride directly to subsecond dopamine release events. We show theoretically that synaptic dopamine release induces low frequency temporal variations of extrasynaptic extracellular dopamine levels, at time scales of one minute, that can evoke detectable temporal variations in the [11C] raclopride signal. Hence, dopaminergic activity can be monitored via temporal fluctuations in the [11C] raclopride PET signal. We validate this theory using fast-scan cyclic voltammetry and [11C] raclopride PET in mice during chemogenetic activation of dopaminergic neurons. We then apply the method to data from human subjects given a palatable milkshake and discover immediate and-for the first time-delayed food-induced dopamine release. This method enables time-dependent regional monitoring of stimulus-evoked dopamine release at physiological levels.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lippert, Rachel N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cremer, Anna Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Thanarajah, Sharmili Edwin UNSPECIFIED UNSPECIFIED UNSPECIFIED Korn, Clio UNSPECIFIED UNSPECIFIED UNSPECIFIED Jahans-Price, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Burgeno, Lauren M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tittgemeyer, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruening, Jens C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Walton, Mark E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Backes, Heiko UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-159091
DOI:	10.1038/s41467-018-08143-4
Journal or Publication Title:	Nat. Commun.
Volume:	10
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VIVO; KINETIC DIVERSITY; RECEPTOR-BINDING; C-11 RACLOPRIDE; HUMAN-BRAIN; PET; LIGAND; NEUROTRANSMISSION; TRANSMISSION; AFFINITY Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15909