Universität zu Köln

Hortmann, M., Walter, J. E., Benning, L., Follo, M., Mayr, R. M., Honegger, U., Robinson, S., Stallmann, D., Duerschmied, D., Twerenbold, R., Badertscher, P., de lavallaz, J. du Fay, Puelacher, C., Bode, C., Ahrens, I. and Mueller, C. (2019). Droplet digital PCR of serum miR-499, miR-21 and miR-208a for the detection of functionally relevant coronary artery disease. Int. J. Cardiol., 275. S. 129 - 136. CLARE: ELSEVIER IRELAND LTD. ISSN 1874-1754

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Abstract

Background: microRNAs (miRNAs) have shown promise as potential new biomarkers for myocardial injury and myocardial ischemia. New digital polymerase chain reaction (PCR) techniques allow for highly precise and reliable absolute direct quantification. Methods: In this pilot study we used droplet digital PCR (ddPCR) to assess if miRNAs might be released into circulation in patients with functionally relevant coronary artery disease (CAD). Blood samples for measurement of high-sensitivity cardiac troponin I (hs-cTnI) and miRNAs were obtained before, immediately after peak stress, and 2 h after stress testing in a blinded manner in consecutive patients referred for rest/stress myocardial perfusion single-photon emission tomography/computer tomography (MPI-SPECT/CT). ddPCR was used to directly quantify the serum concentrations of miR-21, miR-208a, and miR-499 as potential markers of myocardial injury/ischemia. Functionally relevant CAD was determined by expert interpretation of MPI-SPECT/CT, coronary angiography and fractional flow reserve, if performed. Results: Overall, 200 patients were included and functionally relevant CAD was detected in 85 of them (42%). NeithermiR-21, miR-208a, nor miR-499 concentrations differed at rest, stress, or 2-h after stress when comparing patients with versus without functionally relevant CAD, while hs-cTnI concentrations were significantly higher in patients with functionally relevant CAD (P < 0.001). Exercise-induced changes in miRNA or hs-cTnI concentrations did not have diagnostic utility and were similar in patients with versus without functionally relevant CAD. Conclusion: miR-208a, miR-21 and miR-499 concentrations at rest, after exercise and exercise-induced changes do not provide additional clinical value regarding the detection of functionally relevant CAD. (c) 2018 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hortmann, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Walter, J. E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Benning, L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Follo, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mayr, R. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Honegger, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Robinson, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stallmann, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Duerschmied, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Twerenbold, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Badertscher, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED de lavallaz, J. du Fay UNSPECIFIED UNSPECIFIED UNSPECIFIED Puelacher, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bode, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ahrens, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-159283
DOI:	10.1016/j.ijcard.2018.08.031
Journal or Publication Title:	Int. J. Cardiol.
Volume:	275
Page Range:	S. 129 - 136
Date:	2019
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1874-1754
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARDIAC TROPONIN-I; ACUTE EXHAUSTIVE EXERCISE; CIRCULATING MICRORNAS; CARDIOVASCULAR-DISEASE; EARLY-DIAGNOSIS; STRESS TEST; BIOMARKERS; HEART; MANAGEMENT; GUIDELINE Multiple languages Cardiac & Cardiovascular Systems Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15928