Universität zu Köln

Volz, Cornelia, Mirza, Myriam, Langmann, Thomas and Jaegle, Herbert (2019). ERG Alteration Due to the rd8 Mutation of the Crb1 Gene in Cln3 (+/+) (rd8-/rd8-) Mice. S. 395 - 401. CHAM: SPRINGER INTERNATIONAL PUBLISHING AG. ISSN 2214-8019

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Abstract

Mattapallil et al. described that vendor lines for C57BL/6 N mice may carry the rd8 mutation that leads to an ocular phenotype, which could be mistaken for an induced retinal degeneration. This mouse strain is widely used in ophthalmic research as a background for modeling retinal degeneration. In the process of studying Cln3(Delta ex7/8) knock-in mice on a C57BL/6 N background, we became aware of this issue. The aim of this study thus was to use electroretinography to investigate the age-dependent functional loss in Cln3(+/+) (rd8-/rd8-) mice and compare it to C57BL/6 J mice. The scotopic and photopic amplitudes of the a-wave and b-wave decrease significantly in mutant mice with increasing age, and the implicit time is prolonged. Especially the oscillatory potentials arising from inner retinal interaction seem to be notably affected by the rd8 mutation. Surprisingly, the amplitudes in young C57BL/6 J mice were lower than those measured in C57BL/6 N at any time point. Our results indicate that the rd8 mutation present in C57BL/6 N mice affects the function of the inner and outer retina. This is surprising given that the major retinal morphological alterations due to the rd8 mutation are found in the outer retina. We conclude that the rd8 mutation does affect the retinal function in Cln3(+/+ rd8-/rd8-) mice in a variable manner. Epigenetic factors and modifying genes lead to a phenotype shift in these mice. Interpreting the results of previous studies in mutant mice on C57BL/6 N background is challenging as comparing results obtained in independent studies or on other mouse backgrounds may be misleading. Using littermates as controls remains the only valid option.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Volz, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Mirza, Myriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Langmann, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaegle, Herbert UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-159861
DOI:	10.1007/978-3-030-27378-1_65
Title of Book:	Advances in Experimental Medicine and Biology
Series Name:	Adv.Exp.Med.Biol.
Volume:	1185
Page Range:	S. 395 - 401
Date:	2019
Publisher:	SPRINGER INTERNATIONAL PUBLISHING AG
Place of Publication:	CHAM
ISSN:	2214-8019
ISBN:	978-3-030-27378-1; 978-3-030-27377-4
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHENOTYPE Multiple languages Medicine, Research & Experimental; Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15986