Universität zu Köln

Pabst, Stefan ORCID: 0000-0003-1073-4246, Doering, Lennard-Maximilian, Petreska, Natasha and Dohmen, R. Juergen ORCID: 0000-0002-5756-6780 (2019). Methods to study SUMO dynamics in yeast. In: Methods in Enzymology, S. 187 - 211. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC. ISBN 978-0-12-816359-7

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Abstract

Covalent modification of proteins with the small ubiquitin-related modifier (SUMO) is found in all eukaryotes and is involved in many important processes. SUMO attachment may change interaction properties, subcellular localization, or stability of a modified protein. Usually, only a small fraction of a protein is modified at a given time because sumoylation is a highly dynamic process. The sumoylated state of a protein is controlled by the activity of the sumoylation enzymes that promote either their mono- or poly-sumoylation (SUMO chain formation), by SUMO proteases that reverse these modifications, and by SUMO-targeted ubiquitin ligases (STUbL, ULS) that mediate their degradation by the proteasome. While some organisms, such as humans, express multiple isoforms, budding yeast SUMO is encoded by a single and essential gene termed SMT3. The analysis of the simpler SUMO system in budding yeast has been instrumental in the identification of enzymes acting on this modification and controlling its dynamics. Sumoylation of proteins changes dramatically during the cell division cycle and under various stress conditions. Here we summarize various approaches that employ Saccharomyces cerevisiae as a model system to study the dynamics of sumoylation and how it is controlled.

Item Type:	Book Section, Proceedings Item or annotation in a legal commentary
Creators:	Creators Email ORCID ORCID Put Code Pabst, Stefan UNSPECIFIED orcid.org/0000-0003-1073-4246 UNSPECIFIED Doering, Lennard-Maximilian UNSPECIFIED UNSPECIFIED UNSPECIFIED Petreska, Natasha UNSPECIFIED UNSPECIFIED UNSPECIFIED Dohmen, R. Juergen UNSPECIFIED orcid.org/0000-0002-5756-6780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-160229
DOI:	10.1016/bs.mie.2018.12.026
Title of Book:	Methods in Enzymology
Series Name:	Methods Enzymol.
Volume:	618
Page Range:	S. 187 - 211
Date:	2019
Publisher:	ELSEVIER ACADEMIC PRESS INC
Place of Publication:	SAN DIEGO
ISSN:	0076-6879
ISBN:	978-0-12-816359-7
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN SUMOYLATION; CONJUGATION; LIGASE; INSIGHTS; PATHWAY Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16022