Universität zu Köln

Hou, Yanhong, Toth, Gabor, Siebelmann, Sebastian, Horstmann, Jens, Gabriel, Tim, Bock, Felix and Cursiefen, Claus (2018). UV light crosslinking regresses mature corneal blood and lymphatic vessels and promotes subsequent high-risk corneal transplant survival. Am. J. Transplant., 18 (12). S. 2873 - 2885. HOBOKEN: WILEY. ISSN 1600-6143

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Abstract

Immunologic graft rejection is the main complication after corneal transplant into pathologically prevascularized so-called high-risk eyes. The aim of this study was to evaluate whether ultraviolet (UV) light crosslinking can regress pathologic corneal blood and lymphatic vessels and thereby improve subsequent graft survival. Using the murine model of suture-induced corneal neovascularization, we found that corneal crosslinking with UVA light and riboflavin regressed both preexisting blood and lymphatic vessels significantly via induction of apoptosis in vascular endothelial cells. In addition, macrophages and CD45(+) cell counts were significantly reduced. Consistently, corneal crosslinking reduced keratocyte density and corneal thickness without affecting corneal nonvascular endothelial cells, iris, and lens depending on the crosslinking duration. Furthermore, using the murine model of corneal transplant, long-term graft survival was significantly promoted (P < .05) and CD4(+)CD25(+)FoxP3(+) T regulatory cells were upregulated (P < .01) in high-risk eyes preoperatively treated with crosslinking. Our results suggest UV light crosslinking as a novel method to regress both pathologic corneal blood and lymphatic vessels and to reduce CD45(+) inflammatory cells. Furthermore, this study demonstrates for the first time that preoperative corneal crosslinking in prevascularized high-risk eyes can significantly improve subsequent graft survival and may become a promising novel therapy in the clinic.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hou, Yanhong UNSPECIFIED UNSPECIFIED UNSPECIFIED Toth, Gabor UNSPECIFIED UNSPECIFIED UNSPECIFIED Siebelmann, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Horstmann, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Gabriel, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-164610
DOI:	10.1111/ajt.14874
Journal or Publication Title:	Am. J. Transplant.
Volume:	18
Number:	12
Page Range:	S. 2873 - 2885
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1600-6143
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REGULATORY T-CELLS; FINE-NEEDLE DIATHERMY; INFLAMMATORY LYMPHANGIOGENESIS; INFECTIOUS KERATITIS; IMMUNE PRIVILEGE; RIBOFLAVIN; VEGF; NEOVASCULARIZATION; ANGIOGENESIS; MACROPHAGE Multiple languages Surgery; Transplantation Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16461