Universität zu Köln

Samuilov, Sladjana, Brilhaus, Dominik ORCID: 0000-0001-9021-3197, Rademacher, Nadine, Flachbart, Samantha, Arab, Leila, Alfarraj, Saleh, Kuhnert, Franziska, Kopriva, Stanislav ORCID: 0000-0002-7416-6551, Weber, Andreas P. M., Mettler-Altmann, Tabea and Rennenberg, Heinz (2018). The Photorespiratory BOU Gene Mutation Alters Sulfur Assimilation and Its Crosstalk With Carbon and Nitrogen Metabolism in Arabidopsis thaliana. Front. Plant Sci., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

This study was aimed at elucidating the significance of photorespiratory serine (Ser) production for cysteine (Cys) biosynthesis. For this purpose, sulfur (S) metabolism and its crosstalk with nitrogen (N) and carbon (C) metabolism were analyzed in wildtype Arabidopsis and its photorespiratory bou-2 mutant with impaired glycine decarboxylase (GDC) activity. Foliar glycine and Ser contents were enhanced in the mutant at day and night. The high Ser levels in the mutant cannot be explained by transcript abundances of genes of the photorespiratory pathway or two alternative pathways of Ser biosynthesis. Despite enhanced foliar Ser, reduced GDC activity mediated a decline in sulfur flux into major sulfur pools in the mutant, as a result of deregulation of genes of sulfur reduction and assimilation. Still, foliar Cys and glutathione contents in the mutant were enhanced. The use of Cys for methionine and glucosinolates synthesis was reduced in the mutant. Reduced GDC activity in the mutant downregulated Calvin Cycle and nitrogen assimilation genes, upregulated key enzymes of glycolysis and the tricarboxylic acid (TCA) pathway and modified accumulation of sugars and TCA intermediates. Thus, photorespiratory Ser production can be replaced by other metabolic Ser sources, but this replacement deregulates the cross-talk between S, N, and C metabolism.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Samuilov, Sladjana UNSPECIFIED UNSPECIFIED UNSPECIFIED Brilhaus, Dominik UNSPECIFIED orcid.org/0000-0001-9021-3197 UNSPECIFIED Rademacher, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Flachbart, Samantha UNSPECIFIED UNSPECIFIED UNSPECIFIED Arab, Leila UNSPECIFIED UNSPECIFIED UNSPECIFIED Alfarraj, Saleh UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhnert, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mettler-Altmann, Tabea UNSPECIFIED UNSPECIFIED UNSPECIFIED Rennenberg, Heinz UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-165335
DOI:	10.3389/fpls.2018.01709
Journal or Publication Title:	Front. Plant Sci.
Volume:	9
Date:	2018
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; ADENOSINE 5-PHOSPHOSULFATE REDUCTASE; SULFATE ASSIMILATION; GLUTAMATE-DEHYDROGENASE; GLUTATHIONE SYNTHESIS; S-ADENOSYLMETHIONINE; LIGHT REGULATION; BIOSYNTHESIS; PLANTS; METHIONINE Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16533