Universität zu Köln

Probst, Kristina, Stermann, Jacek, von Bomhard, Inga, Etich, Julia ORCID: 0000-0003-3238-6692, Pitzler, Lena, Niehoff, Anja, Bluhm, Bjoern, Xu, Haifeng C., Lang, Philipp A., Chmielewski, Markus, Abken, Hinrich, Blissenbach, Birgit, Machova, Alzbeta, Papadopoulou, Nikoletta and Brachvogel, Bent (2018). Depletion of Collagen IX Alpha1 Impairs Myeloid Cell Function. Stem Cells, 36 (11). S. 1752 - 1764. HOBOKEN: WILEY. ISSN 1549-4918

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Abstract

The trabecular extracellular matrix (ECM) forms a three-dimensional scaffold to stabilize the bone marrow, provide substrates for cell-matrix interactions and retain, present or release signals to modulate hematopoietic stem and progenitor cell development. However, the impact of trabecular ECM components on hematopoiesis has been poorly studied. Using collagen IX alpha1 - deficient (Col9a1((-/-))) mice, we revealed that a lack of collagen IX alpha1 results in a disorganized trabecular network enriched in fibronectin, and in a reduction in myeloid cells, which was accompanied by a decrease in colony-stimulating factor 1 receptor expression on monocytes from the bone marrow. In contrast, B-cell numbers in the bone marrow and T-cell numbers in the thymus remained unchanged. Alterations in the bone marrow microenvironment may not only reduce myeloid cell numbers, but also have long-term implications for myeloid cell function. Mice were infected with Listeria moncytogenes to analyze the function of myeloid cells. In this case, an inadequate macrophage-dependent clearance of bacterial infections was observed in Col9a1((-/-)) mice in vivo. This was mainly caused by an impaired interferon-gamma/tumor necrosis factor-alpha-mediated activation of macrophages. The loss of collagen IX alpha1 therefore destabilizes the trabecular bone network, impairs myeloid cell differentiation, and affects the innate immune response against Listeria. Stem Cells 2018;36:1752-1763

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Probst, Kristina UNSPECIFIED UNSPECIFIED UNSPECIFIED Stermann, Jacek UNSPECIFIED UNSPECIFIED UNSPECIFIED von Bomhard, Inga UNSPECIFIED UNSPECIFIED UNSPECIFIED Etich, Julia UNSPECIFIED orcid.org/0000-0003-3238-6692 UNSPECIFIED Pitzler, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Niehoff, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Bluhm, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Haifeng C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lang, Philipp A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Chmielewski, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Abken, Hinrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Blissenbach, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Machova, Alzbeta UNSPECIFIED UNSPECIFIED UNSPECIFIED Papadopoulou, Nikoletta UNSPECIFIED UNSPECIFIED UNSPECIFIED Brachvogel, Bent UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-167358
DOI:	10.1002/stem.2892
Journal or Publication Title:	Stem Cells
Volume:	36
Number:	11
Page Range:	S. 1752 - 1764
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1549-4918
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEMATOPOIETIC STEM-CELLS; EXTRACELLULAR-MATRIX; LISTERIA-MONOCYTOGENES; PROGENITOR CELLS; IMMUNE-SYSTEM; CARTILAGE; BONE; MICE; MACROPHAGE; DIFFERENTIATION Multiple languages Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16735