Universität zu Köln

Mueller, Rolf, Stumpf, Maria, Wehrstedt, Regina, Sukumaran, Salil K., Karow, Malte A., Marko, Marija, Noegel, Angelika A. and Eichinger, Ludwig ORCID: 0000-0003-1594-6117 (2018). The regulatory subunit phr2AB of Dictyostelium discoideum phosphatase PP2A interacts with the centrosomal protein CEP161, a CDK5RAP2 ortholog. Genes Cells, 23 (10). S. 923 - 932. HOBOKEN: WILEY. ISSN 1365-2443

Full text not available from this repository.

Abstract

phr2AB is the regulatory subunit of the Dictyostelium discoideum phosphatase PP2A and is the ortholog of the human B55 regulatory subunit of PP2A. phr2AB was isolated as a binding partner of the centrosomal protein CEP161, an ortholog of mammalian CDK5RAP2. CEP161 is presumably a phosphoprotein and a component of the Hippo pathway. The interaction site was located in the N-terminal half of CEP161 which encompasses the gamma TURC binding domain in CEP161. This binding domain is responsible for binding of the gamma-tubulin ring complex which allows microtubule nucleation at the centrosome. GFP-tagged phr2AB is diffusely distributed throughout the cell and enriched at the centrosome. Ectopic expression of phr2AB as GFP fusion protein led to multinucleation, aberrant nucleus centrosome ratios and an altered sensitivity to okadaic acid. Some of these features were also affected in cells over-expressing domains of CEP161 and in cells from patients suffering from primary microcephaly, which carried a mutated CDK5RAP2 gene.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mueller, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Stumpf, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Wehrstedt, Regina UNSPECIFIED UNSPECIFIED UNSPECIFIED Sukumaran, Salil K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karow, Malte A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Marko, Marija UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichinger, Ludwig UNSPECIFIED orcid.org/0000-0003-1594-6117 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-171224
DOI:	10.1111/gtc.12637
Journal or Publication Title:	Genes Cells
Volume:	23
Number:	10
Page Range:	S. 923 - 932
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2443
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HIPPO SIGNALING PATHWAY; HEAVY-CHAIN; MASTER REGULATOR; MITOTIC SPINDLE; OKADAIC ACID; PHOSPHORYLATION; MITOSIS; 2A; THREONINE; MUTATIONS Multiple languages Cell Biology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17122