Universität zu Köln

Gerlich, Silke C., Walker, Berkley J., Krueger, Stephan and Kopriva, Stanislav ORCID: 0000-0002-7416-6551 (2018). Sulfate Metabolism in C-4 Flaveria Species Is Controlled by the Root and Connected to Serine Biosynthesis. Plant Physiol., 178 (2). S. 565 - 583. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

The evolution of C-4 photosynthesis led to an increase in carbon assimilation rates and plant growth compared to C-3 photosynthetic plants. This enhanced plant growth, in turn, affects the requirement for soil-derived mineral nutrients. However, mineral plant nutrition has scarcely been considered in connection with C-4 photosynthesis. Sulfur is crucial for plant growth and development, and preliminary studies in the genus Flaveria suggested metabolic differences in sulfate assimilation along the C-4 evolutionary trajectory. Here, we show that in controlled conditions, foliar accumulation of the reduced sulfur compounds Cys and glutathione (GSH) increased with progressing establishment of the C-4 photosynthetic cycle in different Flaveria species. An enhanced demand for reduced sulfur in C-4 Flaveria species is reflected in high rates of [S-35]sulfate incorporation into GSH upon sulfate deprivation and increased GSH turnover as a reaction to the inhibition of GSH synthesis. Expression analyses indicate that the gamma-glutamyl cycle is crucial for the recycling of GSH in C-4 species. Sulfate reduction and GSH synthesis seems to be preferentially localized in the roots of C-4 species, which might be linked to its colocalization with the phosphorylated pathway of Ser biosynthesis. Interspecies grafting experiments of F. robusta (C-3) and F. bidentis (C-4) revealed that the root system primarily controls sulfate acquisition, GSH synthesis, and sulfate and metabolite allocation in C-3 and C-4 plants. This study thus shows that evolution of C-4 photosynthesis resulted in a wide range of adaptations of sulfur metabolism and points out the need for broader studies on importance of mineral nutrition for C-4 plants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gerlich, Silke C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Walker, Berkley J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-171436
DOI:	10.1104/pp.18.00520
Journal or Publication Title:	Plant Physiol.
Volume:	178
Number:	2
Page Range:	S. 565 - 583
Date:	2018
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; X POPULUS-ALBA; ARABIDOPSIS-THALIANA; PHOTOSYNTHETIC ORGANISMS; GLUTATHIONE SYNTHESIS; 3-PHOSPHOGLYCERATE DEHYDROGENASE; PHOSPHORYLATED PATHWAY; SULFUR ASSIMILATION; CELL-PROLIFERATION; GENE-EXPRESSION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17143