Universität zu Köln

Noethel, Barbara, Ramms, Lena, Dreissen, Georg, Hoffmann, Marco, Springer, Ronald, Ruebsam, Matthias, Ziegler, Wolfgang H. ORCID: 0000-0003-4529-1916, Niessen, Carien M., Merkel, Rudolf ORCID: 0000-0003-3178-3282 and Hoffmann, Bernd ORCID: 0000-0002-3803-8835 (2018). Transition of responsive mechanosensitive elements from focal adhesions to adherens junctions on epithelial differentiation. Mol. Biol. Cell, 29 (19). S. 2317 - 2326. BETHESDA: AMER SOC CELL BIOLOGY. ISSN 1939-4586

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Abstract

The skin's epidermis is a multilayered epithelial tissue and the first line of defense against mechanical stress. Its barrier function depends on an integrated assembly and reorganization of cell-matrix and cell-cell junctions in the basal layer and on different intercellular junctions in suprabasal layers. However, how mechanical stress is recognized and which adhesive and cytoskeletal components are involved are poorly understood. Here, we subjected keratinocytes to cyclic stress in the presence or absence of intercellular junctions. Both states not only recognized but also responded to strain by reorienting actin filaments perpendicular to the applied force. Using different keratinocyte mutant strains that altered the mechanical link of the actin cytoskeleton to either cell-matrix or cell-cell junctions, we show that not only focal adhesions but also adherens junctions function as mechanosensitive elements in response to cyclic strain. Loss of paxillin or talin impaired focal adhesion formation and only affected mechanosensitivity in the absence but not presence of intercellular junctions. Further analysis revealed the adherens junction protein a-catenin as a main mechanosensor, with greatest sensitivity conferred on binding to vinculin. Our data reveal a mechanosensitive transition from cell-matrix to cell-cell adhesions on formation of keratinocyte monolayers with vinculin and a-catenin as vital players.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Noethel, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Ramms, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Dreissen, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoffmann, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Springer, Ronald UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruebsam, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Ziegler, Wolfgang H. UNSPECIFIED orcid.org/0000-0003-4529-1916 UNSPECIFIED Niessen, Carien M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Merkel, Rudolf UNSPECIFIED orcid.org/0000-0003-3178-3282 UNSPECIFIED Hoffmann, Bernd UNSPECIFIED orcid.org/0000-0002-3803-8835 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-172871
DOI:	10.1091/mbc.E17-06-0387
Journal or Publication Title:	Mol. Biol. Cell
Volume:	29
Number:	19
Page Range:	S. 2317 - 2326
Date:	2018
Publisher:	AMER SOC CELL BIOLOGY
Place of Publication:	BETHESDA
ISSN:	1939-4586
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ALPHA-CATENIN; E-CADHERIN; DYNAMIC REORIENTATION; VINCULIN ACTIVATION; MECHANICAL-STRESS; FORCE; CELLS; SKIN; MECHANOTRANSDUCTION; ARCHITECTURE Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17287