Universität zu Köln

Ruthard, Johannes, Hermes, Gabriele, Hartmann, Ursula, Sengle, Gerhard, Pongratz, Georg, Ostendorf, Benedikt, Schneider, Matthias, Hoellriegl, Stefan, Zaucke, Frank, Wagener, Raimund, Streichert, Thomas and Klatt, Andreas R. (2018). Identification of antibodies against extracellular matrix proteins in human osteoarthritis. Biochem. Biophys. Res. Commun., 503 (3). S. 1273 - 1278. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2104

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Abstract

We investigated the presence of autoantibodies against the extracellular matrix proteins thrombospondin-4 (TSP-4), cartilage oligomeric matrix protein (COMP), C-type lectin domain family 3 member A (CLEC3A), collagen II, collagen VI, matrilin-3, and fibrillin-2 in the serum of osteoarthritis (OA) patients. We compared those results with the presence of such antibodies in rheumatoid arthritis (RA) patients and in healthy donors (HD). Our study examines whether antibodies against extracellular proteins can be used as potential biomarkers to support the clinical diagnosis of OA. 10 OA, 10 RA patients and 10 HD were enrolled in this explorative cross-sectional study. SDS-PAGE and immunoblot were used to investigate the presence of antibodies against extracellular matrix proteins. The serum of 5/10 OA patients but 0/10 HD exhibited TSP-4 IgG isotype antibodies (P = 0.033). The serum of 8/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4 or COMP (P = 0.005). The serum of 9/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4, COMP or CLEC3A (P = 0.005). We found strong evidence for the presence of IgG isotype autoantibodies against the cartilage extracellular matrix proteins TSP-4, COMP and CLEC3A in OA. The detection of IgG isotype autoantibodies against TSP-4, COMP and CLEC3A may support the clinical diagnosis of OA. OA with autoantibodies against cartilage extracellular matrix proteins defines a new OA subgroup suggesting that patients with high concentrations of autoantibodies may benefit from an immune suppressive therapy. (C) 2018 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ruthard, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Hermes, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Sengle, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Pongratz, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Ostendorf, Benedikt UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoellriegl, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED Streichert, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Klatt, Andreas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-173049
DOI:	10.1016/j.bbrc.2018.07.036
Journal or Publication Title:	Biochem. Biophys. Res. Commun.
Volume:	503
Number:	3
Page Range:	S. 1273 - 1278
Date:	2018
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2104
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYNOVIAL-FLUID; JOINT DISEASE; T-CELLS; KNEE; COMPLEMENT; ARTHRITIS; MICE; IL-6; INTERLEUKIN-6; PROTEOGLYCAN Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17304