Universität zu Köln

Dunaiski, Cara Mia, Janssen, Lena, Erzinger, Hannah, Pieper, Monika, Damaschek, Sarah, Schildgen, Oliver ORCID: 0000-0003-4297-9627 and Schildgen, Verena (2018). Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups. J. Fungi, 4 (3). BASEL: MDPI. ISSN 2309-608X

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Abstract

The molecular detection of Pneumocystis jirovecii is an important therapy-relevant tool in microbiological diagnostics. However, the quantification of this pathogen in the past has revealed discordant results depending on the target gene. As the clinical variety of P. jirovecii infections ranges between life-threatening infections and symptom-free colonization, the question arises if qPCRs are reliable tools for quantitative diagnostics of P. jirovecii. P. jirovecii positive BALs were quantitatively tested for the copy numbers of one mitochondrial (COX-1) and two nuclear single-copy genes (KEX1 and DHPS) compared to the mitochondrial large subunit (mtLSU) by qPCR. Independent of the overall mtLSU copy number P. jirovecii clustered into distinct groups based on the ratio patterns of the respective qPCRs. This study, which compared different mitochondrial to nuclear gene ratio patterns of independent patients, shows that the mtLSU gene represents a highly sensitive qPCR tool for the detection of P. jirovecii, but does not display a reliable target for absolute quantification.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dunaiski, Cara Mia UNSPECIFIED UNSPECIFIED UNSPECIFIED Janssen, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Erzinger, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Pieper, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Damaschek, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-174014
DOI:	10.3390/jof4030084
Journal or Publication Title:	J. Fungi
Volume:	4
Number:	3
Date:	2018
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TIME PCR ASSAY; SERINE ENDOPROTEASE; CARINII-PNEUMONIA; RIBOSOMAL-RNA; COLONIZATION; LUNG; GENOTYPES; SEQUENCE Multiple languages Microbiology; Mycology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17401