Universität zu Köln

Goldammer, Jens ORCID: 0000-0002-5623-8339, Mantziaris, Charalampos ORCID: 0000-0003-2280-1733, Bueschges, Ansgar and Schmidt, Joachim (2018). Calcium imaging of CPG-evoked activity in efferent neurons of the stick insect. PLoS One, 13 (8). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The stick insect is a well-established experimental animal to study the neural basis of walking. Here, we introduce a preparation that allows combining calcium imaging in efferent neurons with electrophysiological recordings of motor neuron activity in the stick insect thoracic nerve cord. The intracellular free calcium concentration in middle leg retractor coxae motor neurons and modulatory octopaminergic DUM neurons was monitored after backfilling lateral nerve nl5 that contains the axons of these neurons with the calcium indicator Oregon Green BAPTA-1. Rhythmic spike activity in retractor and protractor motor neurons was evoked by pharmacological activation of central pattern generating neuronal networks and recorded extracellularly from lateral nerves. A primary goal of this study was to investigate whether changes in the intracellular free calcium concentration observed in motor neurons during oscillatory activity depend on action potentials. We show that rhythmic spike activity in leg motor neurons induced either pharmacologically or by tactile stimulation of the animal is accompanied by a synchronous modulation in the intracellular free calcium concentration. Calcium oscillations in motor neurons do not appear to depend on calcium influx through voltage-sensitive calcium channels that are gated by action potentials because Calcium oscillations persist after pharmacologically blocking action potentials in the motor neurons. Calcium oscillations were also apparent in the modulatory DUM neurons innervating the same leg muscle. However, the timing of calcium oscillations varied not only between DUM neurons and motor neurons, but also among different DUM neurons. Therefore, we conclude that the motor neurons and the different DUM neurons receive independent central drive.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goldammer, Jens UNSPECIFIED orcid.org/0000-0002-5623-8339 UNSPECIFIED Mantziaris, Charalampos UNSPECIFIED orcid.org/0000-0003-2280-1733 UNSPECIFIED Bueschges, Ansgar UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176089
DOI:	10.1371/journal.pone.0202822
Journal or Publication Title:	PLoS One
Volume:	13
Number:	8
Date:	2018
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language UNPAIRED MEDIAN NEURONS; CARAUSIUS-MOROSUS; ELECTRICAL-PROPERTIES; PATTERN GENERATION; MANDUCA-SEXTA; NEUROMODULATORY NEURONS; MOTONEURONAL ACTIVITY; SEARCHING MOVEMENTS; INTRACELLULAR CA2+; PLATEAU POTENTIALS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17608