Universität zu Köln

Notara, M., Behboudifard, S., Kluth, M. A., Masslo, C., Ganss, C., Frank, M. H., Schumacher, B. and Cursiefen, C. (2018). UV light-blocking contact lenses protect against short-term UVB-induced limbal stem cell niche damage and inflammation. Sci Rep, 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

UVB irradiation has been linked to pathogenesis of pterygium, a conjunctival tumor growing onto transparent cornea, the windscreen of the eye. Due to corneal anatomy, ambient UVB irradiation is amplified at the stem cell-containing nasal limbus. The aim of this study was to analyse the effect of a UV-blocking contact lens (UVBCL, senofilcon A, Class 1 UV blocker) on limbal epithelial cells and fibroblasts under UVB irradiation compared to a non-UVB-blocking contact lens. UVBCL prevented UVB-induced DNA damage (as assessed by cyclobutane pyrimidine dimer immunostaining) as well as a decrease in proliferation and scratch wound closure rate of both limbal epithelial and fibroblast cells. Similarly, UVBCL protected limbal epithelial cells from UVB-induced loss of their phenotype in terms of colony forming efficiency and stem cell marker expression (ABCB5, P63 alpha, integrin beta 1) compared to controls. Moreover, with UVBCL pro-inflammatory cytokines such as TNF alpha and MCP1 remained unchanged. These data demonstrate the significance of UV-protection in preserving the limbal niche in response to at least short-term UVB. Our data support the use of UVBCL in protecting limbal niche cells, especially after limbal stem cell transplantation and in patients after pterygium surgery, to help prevent recurrences.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Notara, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Behboudifard, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kluth, M. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Masslo, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ganss, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Frank, M. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schumacher, B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176149
DOI:	10.1038/s41598-018-30021-8
Journal or Publication Title:	Sci Rep
Volume:	8
Date:	2018
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PTERYGIUM EPITHELIAL-CELLS; ENDOTHELIAL GROWTH-FACTOR; ULTRAVIOLET-RADIATION; MEDIATED INDUCTION; IRRADIATION LEADS; PYRIMIDINE DIMERS; INDUCED APOPTOSIS; BASAL CELLS; MOUSE MODEL; CORNEAL Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17614