Universität zu Köln

Rust, Katja ORCID: 0000-0001-6367-5994, Tiwari, Manu D., Mishra, Vivek Kumar ORCID: 0000-0003-0853-0770, Grawe, Ferdi ORCID: 0000-0001-6448-4989 and Wodarz, Andreas ORCID: 0000-0002-0007-6289 (2018). Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila. Embo J., 37 (16). HOBOKEN: WILEY. ISSN 1460-2075

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Abstract

Stem cells establish cortical polarity and divide asymmetrically to simultaneously maintain themselves and generate differentiating offspring cells. Several chromatin modifiers have been identified as stemness factors in mammalian pluripotent stem cells, but whether these factors control stem cell polarity and asymmetric division has not been investigated so far. We addressed this question in Drosophila neural stem cells called neuroblasts. We identified the Tip60 chromatin remodeling complex and its interaction partner Myc as regulators of genes required for neuroblast maintenance. Knockdown of Tip60 complex members results in loss of cortical polarity, symmetric neuroblast division, and premature differentiation through nuclear entry of the transcription factor Prospero. We found that aPKC is the key target gene of Myc and the Tip60 complex subunit Domino in regulating neuroblast polarity. Our transcriptome analysis further showed that Domino regulates the expression of mitotic spindle genes previously identified as direct Myc targets. Our findings reveal an evolutionarily conserved functional link between Myc, the Tip60 complex, and the molecular network controlling cell polarity and asymmetric cell division.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rust, Katja UNSPECIFIED orcid.org/0000-0001-6367-5994 UNSPECIFIED Tiwari, Manu D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mishra, Vivek Kumar UNSPECIFIED orcid.org/0000-0003-0853-0770 UNSPECIFIED Grawe, Ferdi UNSPECIFIED orcid.org/0000-0001-6448-4989 UNSPECIFIED Wodarz, Andreas UNSPECIFIED orcid.org/0000-0002-0007-6289 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176442
DOI:	10.15252/embj.201798659
Journal or Publication Title:	Embo J.
Volume:	37
Number:	16
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1460-2075
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REGULATES SPINDLE ORIENTATION; ASYMMETRIC CELL-DIVISION; SELF-RENEWAL; PROSPERO GENE; C-MYC; ACETYLTRANSFERASE COMPLEX; NEURONAL DIFFERENTIATION; NEURAL PROLIFERATION; ESSENTIAL COFACTOR; CORTICAL POLARITY Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17644