Universität zu Köln

Pietsch, Michael ORCID: 0000-0003-4807-3244, Irrgang, Alexandra ORCID: 0000-0003-1266-9978, Roschanski, Nicole, Michael, Geovana Brenner, Hamprecht, Axel ORCID: 0000-0003-1449-5780, Rieber, Heime, Kaesbohrer, Annemarie, Schwarz, Stefan, Roesler, Uwe, Kreienbrock, Lothar, Pfeifer, Yvonne, Fuchs, Stephan and Werner, Guido (2018). Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany. BMC Genomics, 19. LONDON: BMC. ISSN 1471-2164

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Abstract

Background: Resistance to 3rd-generation cephalosporins in Escherichia coli is mostly mediated by extended-spectrum beta-lactamases (ESBLs) or AmpC beta-lactamases. Besides overexpression of the species-specific chromosomal ampC gene, acquisition of plasmid-encoded ampC genes, e.g. bla(CMY-2), has been described worldwide in E coli from humans and animals. To investigate a possible transmission of bla(CMY-2) along the food production chain, we conducted a next-generation sequencing (NGS)-based analysis of 164 CMY-2-producing E coli isolates from humans, livestock animals and foodstuff from Germany. Results: The data of the 164 sequenced isolates revealed 59 different sequence types (STs); the most prevalent ones were ST38 (n = 19), ST131 (n = 16) and ST117 (n = 13). Two STs were present in all reservoirs: ST131 (human n = 8; food n = 2; animal n = 6) and ST38 (human n = 3; animal n = 9; food n = 7). All but one CMY-2-producing ST131 isolates belonged to the Glade B (fimH22) that differed substantially from the worldwide dominant CTX-M-15-producing clonal lineage ST131-O25b Glade C (fimH30). Plasmid replicon types Inch (n = 61) and IncK (n = 72) were identified for the majority of bla(CMY-2)-carrying plasmids. Plasmid sequence comparisons showed a remarkable sequence identity, especially for IncK plasmids. Associations of replicon types and distinct STs were shown for IncK and ST57, ST429 and ST38 as well as for Incl1 and ST58. Additional beta-lactamase genes (bla(TEM), bla(CTX-M), bla(OXA), bla(SHV)) were detected in 50% of the isolates, and twelve E. coli from chicken and retail chicken meat carried the colistin resistance gene mcr-1. Conclusion: We found isolates of distinct E. coli clonal lineages (ST131 and ST38) in all three reservoirs. However, a direct clonal relationship of isolates from food animals and humans was only noticeable for a few cases. The CMY-2-producing E coli-ST131 represents a clonal lineage different from the CTX-M-15-producing ST131-O25b cluster. Apart from the ST-driven spread, plasmid-mediated spread, especially via Inc1 and IncK plasmids, likely plays an important role for emergence and transmission of bla(CMY-2) between animals and humans.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pietsch, Michael UNSPECIFIED orcid.org/0000-0003-4807-3244 UNSPECIFIED Irrgang, Alexandra UNSPECIFIED orcid.org/0000-0003-1266-9978 UNSPECIFIED Roschanski, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Michael, Geovana Brenner UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED Rieber, Heime UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaesbohrer, Annemarie UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Roesler, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreienbrock, Lothar UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfeifer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuchs, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Werner, Guido UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176580
DOI:	10.1186/s12864-018-4976-3
Journal or Publication Title:	BMC Genomics
Volume:	19
Date:	2018
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1471-2164
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPECTRUM-BETA-LACTAMASE; URINARY-TRACT-INFECTIONS; FATTENING PIG FARMS; SALMONELLA-ENTERICA; OUTBREAK DETECTION; POULTRY MEAT; E. COLI; MOLECULAR CHARACTERIZATION; COMPANION ANIMALS; PLASMID Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17658