Universität zu Köln

Neupert, Susanne, Fusca, Debora, Kloppenburg, Peter and Predel, Reinhard (2018). Analysis of Single Neurons by Perforated Patch Clamp Recordings and MALDI-TOF Mass Spectrometry. ACS Chem. Neurosci., 9 (8). S. 2089 - 2104. WASHINGTON: AMER CHEMICAL SOC. ISSN 1948-7193

Full text not available from this repository.

Abstract

Single-cell mass spectrometry has become an established technique to study specific molecular properties such as the neuropeptide complement of identified neurons. Here, we describe a strategy to characterize, by MALDI-TOF mass spectrometry, neurochemical composition of neurons that were identified by their electrophysiological and neuroanatomical characteristics. The workflow for the first time combined perforated patch clamp recordings with dye loading by electroporation for electrophysiological and neuroanatomical characterization as well as chemical profiling of somata by MALDI-TOF mass spectrometry with subsequent immunohistochemistry. To develop our protocol, we used identified central olfactory neurons from the American cockroach Periplaneta americana. First, the combined approach was optimized using a relative homogeneous, well-characterized neuron population of uniglomerular projection neurons, which show acetylcholine esterase immunoreactivity. The general applicability of this approach was verified on local interneurons, which are a diverse neuron population expressing highly differentiated neuropeptidomes. Thus, this study shows that the newly established protocol is suitable to comprehensively analyze electrophysiological, neuroanatomical, and molecular properties of single neurons. We consider this approach an important step to foster single-cell analysis in a wide variety of neuron types.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Fusca, Debora UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloppenburg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-177000
DOI:	10.1021/acschemneuro.8b00163
Journal or Publication Title:	ACS Chem. Neurosci.
Volume:	9
Number:	8
Page Range:	S. 2089 - 2104
Date:	2018
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1948-7193
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DROSOPHILA ANTENNAL LOBE; COCKROACH PERIPLANETA-AMERICANA; OLFACTORY LOCAL INTERNEURONS; MOTH MANDUCA-SEXTA; IDENTIFIED NEURONS; SYNAPTIC ORGANIZATION; PROJECTION NEURONS; INSECT; CELLS; BRAIN Multiple languages Biochemistry & Molecular Biology; Chemistry, Medicinal; Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17700