Universität zu Köln

Karaiskos, Nikos, Rahmatollahi, Mahdieh, Boltengagen, Anastasiya, Liu, Haiyue, Hoehne, Martin, Rinschen, Markus ORCID: 0000-0002-9252-1342, Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas, Rajewsky, Nikolaus ORCID: 0000-0002-4785-4332, Kocks, Christine, Kann, Martin and Mueller, Roman-Ulrich (2018). A Single-Cell Transcriptome Atlas of the Mouse Glomerulus. J. Am. Soc. Nephrol., 29 (8). S. 2060 - 2069. WASHINGTON: AMER SOC NEPHROLOGY. ISSN 1533-3450

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Abstract

Background Three different cell types constitute the glomerular filter: mesangial cells, endothelial cells, and podocytes. However, to what extent cellular heterogeneity exists within healthy glomerular cell populations remains unknown.Methods We used nanodroplet-based highly parallel transcriptional profiling to characterize the cellular content of purified wild-type mouse glomeruli.Results Unsupervised clustering of nearly 13,000 single-cell transcriptomes identified the three known glomerular cell types. We provide a comprehensive online atlas of gene expression in glomerular cells that can be queried and visualized using an interactive and freely available database. Novel marker genes for all glomerular cell types were identified and supported by immunohistochemistry images obtained from the Human Protein Atlas. Subclustering of endothelial cells revealed a subset of endothelium that expressed marker genes related to endothelial proliferation. By comparison, the podocyte population appeared more homogeneous but contained three smaller, previously unknown subpopulations.Conclusions Our study comprehensively characterized gene expression in individual glomerular cells and sets the stage for the dissection of glomerular function at the single-cell level in health and disease.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Karaiskos, Nikos UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahmatollahi, Mahdieh UNSPECIFIED UNSPECIFIED UNSPECIFIED Boltengagen, Anastasiya UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Haiyue UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoehne, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Rinschen, Markus UNSPECIFIED orcid.org/0000-0002-9252-1342 UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Rajewsky, Nikolaus UNSPECIFIED orcid.org/0000-0002-4785-4332 UNSPECIFIED Kocks, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Kann, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Roman-Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-177316
DOI:	10.1681/ASN.2018030238
Journal or Publication Title:	J. Am. Soc. Nephrol.
Volume:	29
Number:	8
Page Range:	S. 2060 - 2069
Date:	2018
Publisher:	AMER SOC NEPHROLOGY
Place of Publication:	WASHINGTON
ISSN:	1533-3450
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENE-EXPRESSION; FILTRATION; PODOCYTE; REVEALS; ACTIVATION; RESOLUTION; PATHWAY; MICE Multiple languages Urology & Nephrology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17731