Universität zu Köln

Hanisch, Franz-Georg, Hansman, Grant S., Morozov, Vasily, Kunz, Clemens and Schroten, Horst (2018). Avidity of alpha-fucose on human milk oligosaccharides and blood group-unrelated oligo/polyfucoses is essential for potent norovirus-binding targets. J. Biol. Chem., 293 (30). S. 11955 - 11966. BETHESDA: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1083-351X

Full text not available from this repository.

Abstract

There is agreement with respect to norovirus infection routes in humans regarding binding of the pathogen to gastrointestinal epithelia via recognition of blood group-active mucin-typeO-glycans as the initiating and essential event. Among food additives playing a potential role in applications to protect newborns, human milk oligosaccharides (HMOs) as competitors are of major importance. By focusing on fractions of high-molecular mass HMOs with high fucose contents, we attempted to identify the structural elements required for norovirus GII.4 (Sydney 2012, JX459908) capsid binding in neoglycolipid-based arrays. We provide evidence that HMO fractions with the strongest binding capacities contained hepta- to decasaccharides expressing branches with terminal blood group H1 or Lewis-b antigen. H2 antigen, as recognized by UEA-I lectin, is apparently not expressed in high-mass HMOs. Beyond affinity, sterical and valency effects contribute more to virus-like particle binding, as revealed for oligovalent fucose conjugates of alpha-cyclodextrin and oligofucoses from fucoidan. Accordingly, high-mass HMOs with oligovalent fucose can exhibit stronger binding capacities compared with monovalent fucose HMOs. The above features were revealed for the most clinically relevant and prevalent GII.4 strain and are distinct from other strains, like GII.10 (Vietnam 026, AF504671), which showed a preference for blood group Lewis-a positive glycans.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hanisch, Franz-Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hansman, Grant S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morozov, Vasily UNSPECIFIED UNSPECIFIED UNSPECIFIED Kunz, Clemens UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroten, Horst UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-179449
DOI:	10.1074/jbc.RA117.001369
Journal or Publication Title:	J. Biol. Chem.
Volume:	293
Number:	30
Page Range:	S. 11955 - 11966
Date:	2018
Publisher:	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication:	BETHESDA
ISSN:	1083-351X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GROUP ANTIGENS; HELICOBACTER-PYLORI; INFECTION; GASTROENTERITIS; NOMENCLATURE; INHIBITION; DISCOVERY Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17944