Universität zu Köln

Chiorean, Roxana, Danescu, Sorina, Virtic, Oana, Mustafa, Mayson B., Baican, Adrian, Lischka, Annette, Hashimoto, Takashi, Kariya, Yoshinobu, Koch, Manuel and Sitaru, Cassian ORCID: 0000-0002-0370-1270 (2018). Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid. Orphanet J. Rare Dis., 13. LONDON: BMC. ISSN 1750-1172

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Abstract

Background: Mucous membrane pemphigoid is a group of chronic subepithelial autoimmune blistering diseases that mainly affect mucous membranes. Laminin 332-specific autoantibodies are present in approximately 1/3 of the patients, being associated with an increased risk of malignancy. Because of the severe complications, an early recognition of the disease allowing a timely therapy is essential. The gold standard methods for detection of laminin 332-specific autoantibodies, including the immunoprecipitation and immunoblotting are non-quantitative, laborious and restricted to a few specialized laboratories worldwide. In addition, the use of radioimmunoassays, although highly sensitive and specific, are laborious, expensive and tightly regulated. Therefore, there is a stringent need for a quantitative immunoassay for the routine detection of laminin 332-specific autoantibodies more broadly available to diagnostic laboratories. The aim of this study was to compare different antigenic substrates, including native, recombinant laminin 332 and laminin 332-rich keratinocyte extracellular matrix, for development of an ELISA to detect autoantibodies in mucous membrane pemphigoid. Results: Using a relatively large number of sera from MMP patients with well-characterized autoantibody reactivity we show the suitability of ELISA systems using laminin 332 preparations as adjunct diagnostic tools in MMP. While glycosylation of laminin 332 does not appear to influence its recognition by MMP autoantibodies, ELISA systems using both purified, native and recombinant laminin 332 demonstrated a high sensitivity and good correlation with the detection of autoantibodies by immunoblotting. ELISA systems using different laminin 332 preparations represent a feasible and more accessible alternative for a broad range of laboratories. Conclusions: Our findings qualify the use of immunoassays with the laminin 332-rich preparations as an ancillary diagnostic tool in mucous membrane pemphigoid.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chiorean, Roxana UNSPECIFIED UNSPECIFIED UNSPECIFIED Danescu, Sorina UNSPECIFIED UNSPECIFIED UNSPECIFIED Virtic, Oana UNSPECIFIED UNSPECIFIED UNSPECIFIED Mustafa, Mayson B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baican, Adrian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lischka, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Hashimoto, Takashi UNSPECIFIED UNSPECIFIED UNSPECIFIED Kariya, Yoshinobu UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Sitaru, Cassian UNSPECIFIED orcid.org/0000-0002-0370-1270 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-179895
DOI:	10.1186/s13023-018-0855-x
Journal or Publication Title:	Orphanet J. Rare Dis.
Volume:	13
Date:	2018
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1750-1172
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPIDERMOLYSIS-BULLOSA-ACQUISITA; AUTOIMMUNE SKIN DISEASES; SUBEPIDERMAL BLISTERS; NEONATAL MICE; AUTOANTIBODIES; TARGET; IGG; GLYCOSYLATION; ANTIGEN-180; PROTEIN Multiple languages Genetics & Heredity; Medicine, Research & Experimental Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17989