Universität zu Köln

Eisenhut, Marion, Hoecker, Natalie, Schmidt, Sidsel Birkelund ORCID: 0000-0002-4193-4454, Basgaran, Rubek Merina, Flachbart, Samantha, Jahns, Peter, Eser, Tabea, Geimer, Stefan, Husted, Soren ORCID: 0000-0003-2020-1902, Webers, Andreas P. M., Leister, Dario ORCID: 0000-0003-1897-8421 and Schneider, Anja (2018). The Plastid Envelope CHLOROPLAST MANGANESE TRANSPORTER1 Is Essential for Manganese Homeostasis in Arabidopsis. Mol. Plant., 11 (7). S. 955 - 970. CAMBRIDGE: CELL PRESS. ISSN 1752-9867

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Abstract

The transition metal manganese (Mn) is indispensable for photoautotrophic growth since photosystem II (PSII) employs an inorganic Mn4CaO5 cluster for water splitting. Here, we show that the Arabidopsis membrane protein CHLOROPLAST MANGANESE TRANSPORTER1 (CMT1) is involved in chloroplast Mn homeostasis. CMT1 is the closest homolog of the previously characterized thylakoid Mn transporter PHOTOSYNTHESIS-AFFECTED MUTANT71 (PAM71). In contrast to PAM71, CMT1 resides at the chloroplast envelope and is ubiquitously expressed. Nonetheless, like PAM71, the expression of CMT1 can also alleviate the Mn-sensitive phenotype of yeast mutant Delta pmr1. The cmt1 mutant is severely suppressed in growth, chloroplast ultrastructure, and PSII activity owing to a decrease in the amounts of pigments and thylakoid membrane proteins. The importance of CMT1 for chloroplast Mn homeostasis is demonstrated by the significant reduction in chloroplast Mn concentrations in cmt1-1, which exhibited reduced Mn binding in PSII complexes. Moreover, CMT1 expression is downregulated in Mn-surplus conditions. The pam71 cmt1-1double mutant resembles the cmt1-1 single mutant rather than pam71 in most respects. Taken together, our results suggest that CMT1 mediates Mn-2(+) uptake into the chloroplast stroma, and that CMT1 and PAM71 function sequentially in Mn delivery to PSII across the chloroplast envelope and the thylakoid membrane.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eisenhut, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Natalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Sidsel Birkelund UNSPECIFIED orcid.org/0000-0002-4193-4454 UNSPECIFIED Basgaran, Rubek Merina UNSPECIFIED UNSPECIFIED UNSPECIFIED Flachbart, Samantha UNSPECIFIED UNSPECIFIED UNSPECIFIED Jahns, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Eser, Tabea UNSPECIFIED UNSPECIFIED UNSPECIFIED Geimer, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Husted, Soren UNSPECIFIED orcid.org/0000-0003-2020-1902 UNSPECIFIED Webers, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leister, Dario UNSPECIFIED orcid.org/0000-0003-1897-8421 UNSPECIFIED Schneider, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-180060
DOI:	10.1016/j.molp.2018.04.008
Journal or Publication Title:	Mol. Plant.
Volume:	11
Number:	7
Page Range:	S. 955 - 970
Date:	2018
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1752-9867
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EVOLVING PHOTOSYSTEM-II; SUPEROXIDE-DISMUTASE; SECRETORY PATHWAY; REDOX HOMEOSTASIS; REPEAT PROTEIN; HIGHER-PLANTS; 1.9 ANGSTROM; ROOT-GROWTH; THALIANA; DEFICIENCY Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18006