Universität zu Köln

Benning, Louise, Robinson, Samuel, Follo, Marie, Heger, Lukas Andreas, Stallmann, Daniela, Duerschmied, Daniel ORCID: 0000-0001-5249-4012, Bode, Christoph, Ahrens, Ingo and Hortmann, Marcus (2018). Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease. J. Vis. Exp. (137). CAMBRIDGE: JOURNAL OF VISUALIZED EXPERIMENTS. ISSN 1940-087X

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Abstract

Circulating serum microRNAs (miRNAs) have shown promise as biomarkers for the cardiovascular disease and acute myocardial infarction (AMI), being released from the cardiovascular cells into the circulation. Circulating miRNAs are highly stable and can be quantified. The quantitative expression of specific miRNAs can be linked to the pathology, and some miRNAs show high tissue and disease specificity. Finding novel biomarkers for cardiovascular diseases is of importance for medical research. Quite recently, digital polymerase chain reaction (dPCR) has been invented. dPCR, combined with fluorescent hydrolysis probes, enables specific direct absolute quantification. dPCR exhibits superior technical qualities, including a low variability, high linearity, and high sensitivity compared to the quantitative polymerase chain reaction (qPCR). Thus, dPCR is a more accurate and reproducible method for directly quantifying miRNAs, particularly for the use in large multi-center cardiovascular clinical trials. In this publication, we describe how to effectively perform digital PCR in order to assess the absolute copy number in serum samples.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Benning, Louise UNSPECIFIED UNSPECIFIED UNSPECIFIED Robinson, Samuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Follo, Marie UNSPECIFIED UNSPECIFIED UNSPECIFIED Heger, Lukas Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Stallmann, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Duerschmied, Daniel UNSPECIFIED orcid.org/0000-0001-5249-4012 UNSPECIFIED Bode, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Ahrens, Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Hortmann, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-180946
DOI:	10.3791/57950
Journal or Publication Title:	J. Vis. Exp.
Number:	137
Date:	2018
Publisher:	JOURNAL OF VISUALIZED EXPERIMENTS
Place of Publication:	CAMBRIDGE
ISSN:	1940-087X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BIOMARKERS; QUANTIFICATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18094