Universität zu Köln

Reina, Jose, Gottardo, Marco ORCID: 0000-0001-8649-7799, Riparbelli, Maria G., Llamazares, Salud, Callaini, Giuliano ORCID: 0000-0003-2252-0309 and Gonzalez, Cayetano ORCID: 0000-0002-5259-3608 (2018). Centrobin is essential for C-tubule assembly and flagellum development in Drosophila melanogaster spermatogenesis. J. Cell Biol., 217 (7). S. 2365 - 2373. NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 1540-8140

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Abstract

Centrobin homologues identified in different species localize on daughter centrioles. In Drosophila melanogaster sensory neurons, Centrobin (referred to as CNB in Drosophila) inhibits basal body function. These data open the question of CNB's role in spermatocytes, where daughter and mother centrioles become basal bodies. In this study, we report that in these cells, CNB localizes equally to mother and daughter centrioles and is essential for C-tubules to attain the right position and remain attached to B-tubules as well as for centrioles to grow in length. CNB appears to be dispensable for meiosis, but flagellum development is severely compromised in Cnb mutant males. Remarkably, three N-terminal POLO phosphorylation sites that are critical for CNB function in neuroblasts are dispensable for spermatogenesis. Our results underpin the multifunctional nature of CNB that plays different roles in different cell types in Drosophila, and they identify CNB as an essential component for C-tubule assembly and flagellum development in Drosophila spermatogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reina, Jose UNSPECIFIED UNSPECIFIED UNSPECIFIED Gottardo, Marco UNSPECIFIED orcid.org/0000-0001-8649-7799 UNSPECIFIED Riparbelli, Maria G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Llamazares, Salud UNSPECIFIED UNSPECIFIED UNSPECIFIED Callaini, Giuliano UNSPECIFIED orcid.org/0000-0003-2252-0309 UNSPECIFIED Gonzalez, Cayetano UNSPECIFIED orcid.org/0000-0002-5259-3608 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-181688
DOI:	10.1083/jcb.201801032
Journal or Publication Title:	J. Cell Biol.
Volume:	217
Number:	7
Page Range:	S. 2365 - 2373
Date:	2018
Publisher:	ROCKEFELLER UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1540-8140
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CENTRIOLE DUPLICATION; PRIMARY CILIA; BASAL BODIES; STEM-CELLS; PROTEIN; CENTROSOMES; SPERMATIDS; SPERMATOCYTE; BLD10/CEP135; NEUROBLASTS Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18168