Universität zu Köln

Wagner, Isabel Viola, Sahlin, Lena, Savchuk, Iuliia, Kloeting, Nora, Svechnikov, Konstantin and Soeder, Olle (2018). Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats. Obesity, 26 (7). S. 1161 - 1168. HOBOKEN: WILEY. ISSN 1930-739X

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Abstract

ObjectiveObesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats. MethodsGene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin-layer chromatography. ResultsSubstantial amounts of testosterone in adipose tissue (25-153 ng/g tissue) and in the supernatant of adipocytes (0.33-0.69 ng/ten thousand cells]) were found. StAR and steroidogenic enzymes encoded by genes including Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3, and Srd5a2 were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue. ConclusionsThe whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wagner, Isabel Viola UNSPECIFIED UNSPECIFIED UNSPECIFIED Sahlin, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Savchuk, Iuliia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloeting, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Svechnikov, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Soeder, Olle UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-182157
DOI:	10.1002/oby.22198
Journal or Publication Title:	Obesity
Volume:	26
Number:	7
Page Range:	S. 1161 - 1168
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1930-739X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYCYSTIC-OVARY-SYNDROME; THECA CELLS; CYTOCHROME-P450 17-ALPHA-HYDROXYLASE; INSULIN-RESISTANCE; METABOLIC PHENOTYPE; ANDROGEN PRODUCTION; WOMEN; METAANALYSIS; PREVALENCE; RECEPTOR Multiple languages Endocrinology & Metabolism; Nutrition & Dietetics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18215