Trusch, Franziska ORCID: 0000-0003-0608-202X, Loebach, Lars, Wawra, Stephan ORCID: 0000-0001-8555-1618, Durward, Elaine, Wuensch, Andreas, Iberahim, Nurul Aqilah, De Bruijn, Irene, MacKenzie, Kevin ORCID: 0000-0002-0041-5375, Willems, Ariane, Toloczko, Aleksandra, Dieguez-Uribeondo, Javier, Rasmussen, Tim ORCID: 0000-0003-1126-846X, Schrader, Thomas, Bayer, Peter ORCID: 0000-0003-0435-7202, Secombes, Chris J. and van West, Pieter ORCID: 0000-0002-0767-6017 (2018). Cell entry of a host-targeting protein of oomycetes requires gp96. Nat. Commun., 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

The animal-pathogenic oomycete Saprolegnia parasitica causes serious losses in aquaculture by infecting and killing freshwater fish. Like plant-pathogenic oomycetes, S. parasitica employs similar infection structures and secretes effector proteins that translocate into host cells to manipulate the host. Here, we show that the host-targeting protein SpHtp3 enters fish cells in a pathogen-independent manner. This uptake process is guided by a gp96-like receptor and can be inhibited by supramolecular tweezers. The C-terminus of SpHtp3 (containing the amino acid sequence YKARK), and not the N-terminal RxLR motif, is responsible for the uptake into host cells. Following translocation, SpHtp3 is released from vesicles into the cytoplasm by another host-targeting protein where it degrades nucleic acids. The effector translocation mechanism described here, is potentially also relevant for other pathogen-host interactions as gp96 is found in both animals and plants.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Trusch, FranziskaUNSPECIFIEDorcid.org/0000-0003-0608-202XUNSPECIFIED
Loebach, LarsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wawra, StephanUNSPECIFIEDorcid.org/0000-0001-8555-1618UNSPECIFIED
Durward, ElaineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wuensch, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Iberahim, Nurul AqilahUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
De Bruijn, IreneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
MacKenzie, KevinUNSPECIFIEDorcid.org/0000-0002-0041-5375UNSPECIFIED
Willems, ArianeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Toloczko, AleksandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dieguez-Uribeondo, JavierUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rasmussen, TimUNSPECIFIEDorcid.org/0000-0003-1126-846XUNSPECIFIED
Schrader, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bayer, PeterUNSPECIFIEDorcid.org/0000-0003-0435-7202UNSPECIFIED
Secombes, Chris J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van West, PieterUNSPECIFIEDorcid.org/0000-0002-0767-6017UNSPECIFIED
URN: urn:nbn:de:hbz:38-182840
DOI: 10.1038/s41467-018-04796-3
Journal or Publication Title: Nat. Commun.
Volume: 9
Date: 2018
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HEAT-SHOCK-PROTEIN; RXLR EFFECTOR AVR3A; PHYTOPHTHORA-INFESTANS; ENDOPLASMIC-RETICULUM; SURFACE EXPRESSION; MEMBRANE-PROTEIN; PLANT-CELLS; PHOSPHATIDYLINOSITOL 3-PHOSPHATE; DISEASE RESISTANCE; PLASMA-MEMBRANEMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18284

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