Annibal, Andrea ORCID: 0000-0003-4311-5417, Karalay, Oezlem, Latza, Christian and Antebi, Adam (2018). A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegans. Anal. Methods, 10 (22). S. 2551 - 2560. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 1759-9679

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Abstract

Endocannabinoids and related N-acyl ethanolamine-derived lipids affect a diverse array of physiological processes and pathological conditions. In the roundworm C. elegans, several endocannabinoid-like molecules have been implicated in regulating axon regeneration, energy balance and food intake as well as aging. One such molecule oleoylethanolamine (OEA) has been shown to promote life extension through nuclear receptor signal transduction, and its accurate quantitation therefore is of high interest. Using a combination of electron impact ionization (EI) and collision induced dissociation coupled to gas chromatography (GC), we found unique fragmentation ions of OEA and designed a specific MRM method for its accurate quantification. Our method should provide a reproducible and robust way to measure OEA dynamics under different genetic, pharmacologic and environmental perturbations.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Annibal, AndreaUNSPECIFIEDorcid.org/0000-0003-4311-5417UNSPECIFIED
Karalay, OezlemUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Latza, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Antebi, AdamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-182876
DOI: 10.1039/c8ay00396c
Journal or Publication Title: Anal. Methods
Volume: 10
Number: 22
Page Range: S. 2551 - 2560
Date: 2018
Publisher: ROYAL SOC CHEMISTRY
Place of Publication: CAMBRIDGE
ISSN: 1759-9679
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHROMATOGRAPHY-MASS-SPECTROMETRY; CAENORHABDITIS-ELEGANS; GAS-CHROMATOGRAPHY; CANNABINOID RECEPTOR; FATTY-ACIDS; LIFE-SPAN; TRIMETHYLSILYL DERIVATIVES; N-OLEOYLETHANOLAMINE; LIPID-METABOLISM; HYDROLASE FAAHMultiple languages
Chemistry, Analytical; Food Science & Technology; SpectroscopyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18287

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