Universität zu Köln

Schroeder, Daniel C., Maul, Alexandra C., Mahabir, Esther, Koxholt, Isabell, Yan, Xiaowei, Padosch, Stephan A., Herff, Holger, Bultmann-Mellin, Insa, Sterner-Kock, Anja, Annecke, Thorsten ORCID: 0000-0002-2496-4432, Hucho, Tim ORCID: 0000-0002-4147-9308, Boettiger, Bernd W. and Guschlbauer, Maria ORCID: 0000-0002-5401-4542 (2018). Evaluation of small intestinal damage in a rat model of 6 Minutes cardiac arrest. BMC Anesthesiol., 18. LONDON: BIOMED CENTRAL LTD. ISSN 1471-2253

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Abstract

Background: Contribution of the small intestine to systemic inflammation after cardiac arrest (CA) is poorly understood. The objective was to evaluate whether an in vivo rat model of 6 min CA is suitable to initiate intestinal ischaemia-reperfusion-injury and to evaluate histomorphological changes and inflammatory processes in the small intestinal mucosa resp. in sera. Methods: Adult male Wistar rats were subjected to CA followed by cardio-pulmonary resuscitation. Proximal jejunum and serum was collected at 6 h, 24 h, 72 h and 7 d post return of spontaneous circulation (ROSC) and from a control group. The small intestine was evaluated histomorphologically. Cytokine concentrations were measured in jejunum lysates and sera. Results: Histomorphological evaluation revealed a significant increase in mucosal damage in the jejunum at all timepoints compared to controls (p < 0.0001). In jejunal tissues, concentrations of IL-1 alpha, IL-1 beta, IL-10, and TNF-alpha showed significant peaks at 24 h and were 1.5- to 5.7-fold higher than concentrations at 6 h and in the controls (p < 0.05). In serum, a significant higher amount of cytokine was detected only for IL-1 beta at 24 h post-ROSC compared to controls (15.78 vs. 9.76 pg/ml). Conclusion: CA resulted in mild small intestinal tissue damage but not in systemic inflammation. A rat model of 6 min CA is not capable to comprehensively mimic a post cardiac arrest syndrome (PCAS). Whether there is a vital influence of the intestine on the PCAS still remains unclear.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schroeder, Daniel C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maul, Alexandra C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahabir, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Koxholt, Isabell UNSPECIFIED UNSPECIFIED UNSPECIFIED Yan, Xiaowei UNSPECIFIED UNSPECIFIED UNSPECIFIED Padosch, Stephan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Herff, Holger UNSPECIFIED UNSPECIFIED UNSPECIFIED Bultmann-Mellin, Insa UNSPECIFIED UNSPECIFIED UNSPECIFIED Sterner-Kock, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Annecke, Thorsten UNSPECIFIED orcid.org/0000-0002-2496-4432 UNSPECIFIED Hucho, Tim UNSPECIFIED orcid.org/0000-0002-4147-9308 UNSPECIFIED Boettiger, Bernd W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Guschlbauer, Maria UNSPECIFIED orcid.org/0000-0002-5401-4542 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-183036
DOI:	10.1186/s12871-018-0530-8
Journal or Publication Title:	BMC Anesthesiol.
Volume:	18
Date:	2018
Publisher:	BIOMED CENTRAL LTD
Place of Publication:	LONDON
ISSN:	1471-2253
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ISCHEMIA-REPERFUSION-INJURY; JEJUNAL SMOOTH-MUSCLE; CEREBRAL-BLOOD-FLOW; CARDIOPULMONARY-RESUSCITATION; STERILE INFLAMMATION; ANIMAL-MODELS; PATHOPHYSIOLOGY; CONTRACTILITY; DYSFUNCTION; HYPOTHERMIA Multiple languages Anesthesiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18303